N-乙酰半胱氨酸对非酒精性脂肪肝病大鼠肝组织NF-κB和TNF-α表达的影响

发布时间：2018-04-19 15:01

本文选题：N-乙酰半胱氨酸 + 非酒精性脂肪肝炎　；参考：《中华中医药学刊》2017年01期

【摘要】：目的:探讨核转录因子-κB(nuclear factor-kappa B,NF-κB)及肿瘤坏死因子α(TNF-α)在非酒精性脂肪性肝病(nonalcoholic fatty liver disease,NAFLD)发病机制中的作用及N-乙酰半胱氨酸(N-acetylcysteine,NAC)的疗效。方法:高脂饮食12周构建NAFLD大鼠模型,模型构建成功后分为NAC低、中、高剂量组、多烯磷脂酰胆碱组和模型组。药物干预12周后,检测肝组织NF-κB、TNF-α蛋白和含量表达情况,检测血清天门冬氨酸转移酶(AST)、丙氨酸氨基转移酶(ALT)、胆固醇(CHOL)、甘油三酯(TG)、空腹血糖(GLU)表达水平和观察大鼠肝脏病理学改变情况。结果:与正常组比较,模型组NF-κB、TNF-α、AST、ALT、CHOL、TG、GLU水平显著升高(P0.05),肝脏病理学检查显示炎症改变和脂肪变性。NAC干预后,与模型组比较,药物组NF-κB、TNF-α、AST、ALT、CHOL、TG、GLU水平显著下降,肝脏病理脂肪变、炎症坏死程度减轻,NAC高剂量组改善最明显。结论:在NAFLD大鼠模型中肝组织NF-κB、TNF-α表达增高,而NAC可能是通过下调NF-κB、TNF-α的表达,起到抗炎作用,从而改善肝脏组织病理学改变。
[Abstract]:Objective: to investigate the role of nuclear factor- 魏 B(nuclear factor-kappa (NF- 魏 B) and tumor necrosis factor- 伪 (TNF- 伪) in the pathogenesis of nonalcoholic fatty liver disease (NAFLD) and the efficacy of N-acetylcysteine NAC- (N-acetylcysteine NAC-) in the pathogenesis of nonalcoholic fatty liver disease.Methods: NAFLD rat model was established after 12 weeks of high fat diet. The model was divided into three groups: low, middle and high dose of NAC, polyene phosphatidylcholine group and model group.After 12 weeks of drug intervention, the protein and content of NF- 魏 B TNF- 伪 in liver tissue were detected.Serum aspartate transferase (AST), alanine aminotransferase (alanine aminotransferase) alanine aminotransferase (alt), cholesterol Choll, triglyceride (TGN), fasting blood glucose (GLU) were detected and the pathological changes of rat liver were observed.Results: compared with the normal group, the level of TNF- 魏 B TNF- 伪 -ASTTURCHOLTGN GLU in the model group was significantly higher than that in the control group. The pathological examination of liver showed that after the intervention of inflammation and fatty degeneration. NAC, compared with the model group, the level of NF- 魏 BAN-TNF- 伪 ASTALTOLCHOLTGGN GLU decreased significantly, and the hepatic pathological fat changed, and compared with the model group, the level of NF- 魏 B TNF- 伪 ASTALTOLCHOLTGGU decreased significantly.The degree of inflammation and necrosis was alleviated most obviously in the high dose group of NAC.Conclusion: in NAFLD rat model, the expression of NF- 魏 BnTNF- 伪 in liver tissue is increased, while NAC may play an anti-inflammatory role by down-regulating the expression of NF- 魏 B- TNF- 伪, thus improving the pathological changes of liver tissue.
【作者单位】：浙江中医药大学附属杭州市西溪医院;杭州市儿童医院;
【基金】：杭州市科技发展计划项目(20130633B09,20140633B29,20110833B17)
【分类号】：R575

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