牛磺酸联合脐带间充质干细胞移植修复酒精性肝损伤

发布时间：2018-04-21 04:59

本文选题：脐带 + 间质干细胞移植　；参考：《中国组织工程研究》2017年01期

【摘要】：背景:研究表明,脐带间充质干细胞可以诱导分化成肝样细胞,从而对肝损伤有一定的修复作用。目的:探讨牛磺酸与人脐带间充质干细胞移植联合治疗对肝损伤的修复作用。方法:48只健康SD大鼠给予56度白酒灌胃30 d建立慢性酒精性肝损伤模型,建模后将其随机分为模型组,脐带间充质干细胞组及牛磺酸+脐带间充质干细胞组。模型组门静脉注射生理盐水1 m L,脐带间充质干细胞组门静脉移植1 mL脐带间充质干细胞悬液,牛磺酸+脐带间充质干细胞组门静脉注射牛磺酸0.28 g/L+1 m L脐带间充质干细胞悬液。移植后2周,检测各组大鼠血清超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶、丙氨酸氨基转移酶以及丙二醛、内毒素、白细胞介素4水平;采用RT-PCR方法检测肝细胞特征性功能蛋白CYP2B1的mRNA表达。结果与结论:(1)与模型组相比较,脐带间充质干细胞组、牛磺酸+脐带间充质干细胞组超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶、谷丙转氨酶水平均显著增高(P0.05),丙二醛、内毒素、白细胞介素4水平均显著下降(P0.05)。与脐带间充质干细胞组比较,牛磺酸+脐带间充质干细胞组超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶、谷丙转氨酶水平进一步增高(P0.05),丙二醛、内毒素、白细胞介素4水平进一步下降(P0.05);(2)与模型组、脐带间充质干细胞组比较,肝细胞特征性功能蛋白CYP2B1的mRNA表达在牛磺酸+脐带间充质干细胞组显著增高,差异有显著性意义(P0.05);(3)以上实验结果表明,脐带间充质干细胞移植可以修复大鼠肝损伤,与牛磺酸联合使用后效果更佳,其可能与减少自由基对机体的损害有关。
[Abstract]:Background: umbilical cord mesenchymal stem cells can induce hepatoid cells and repair liver injury. Objective: to investigate the repair effect of taurine combined with human umbilical cord mesenchymal stem cell transplantation on liver injury. Methods 48 healthy Sprague-Dawley rats were given 56 degrees liquor for 30 days to establish the model of chronic alcoholic liver injury. The model group was randomly divided into three groups: umbilical cord mesenchymal stem cell group and taurine umbilical cord mesenchymal stem cell group. In model group, 1 mL of umbilical cord mesenchymal stem cell suspension was injected into portal vein and 1 mL of umbilical cord mesenchymal stem cell was injected into portal vein of model group and umbilical cord mesenchymal stem cell group. Mesenchymal stem cells were injected with taurine 0.28 g / L -1 mL umbilical cord mesenchymal stem cell suspension via portal vein. The levels of serum superoxide dismutase, glutathione peroxidase, catalase, alanine aminotransferase, malondialdehyde, endotoxin and interleukin-4 were measured 2 weeks after transplantation. The mRNA expression of CYP2B1 was detected by RT-PCR. Results compared with the model group, superoxide dismutase (SOD), glutathione peroxidase (GSH) and catalase were found in the cord mesenchymal stem cell group and taurine cord mesenchymal stem cell group. The levels of glutamate-alanine aminotransferase (alt) increased significantly (P 0.05), and the levels of malondialdehyde (MDA), endotoxin (LPS) and interleukin 4 (IL-4) decreased significantly (P 0.05). Compared with the umbilical cord mesenchymal stem cells group, the levels of superoxide dismutase, glutathione peroxidase, catalase and glutamate-alanine aminotransferase in taurine cord mesenchymal stem cell group were further increased. Compared with model group and umbilical cord mesenchymal stem cell group, the mRNA expression of hepatocyte characteristic protein CYP2B1 was significantly increased in taurine umbilical cord mesenchymal stem cell group. The results showed that the transplantation of umbilical cord mesenchymal stem cells could repair liver injury in rats, and the effect of combined treatment with taurine was better, which might be related to reducing the damage of free radicals to the body.
【作者单位】：河北省遵化市人民医院外一科;河北省遵化市人民医院病理科;
【分类号】：R575

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