转化生长因子-β通路在腹腔间隔室综合征大鼠模型肠黏膜损伤修复中的作用

发布时间：2018-04-23 16:30

本文选题：腹内高压 + 腹腔间隔室综合征　；参考：《广东医学》2017年24期

【摘要】：目的探讨转化生长因子-β(TGF-β)信号通路在腹腔间隔室综合征(ACS)大鼠模型肠黏膜损伤修复中的作用。方法 SD大鼠30只,随机分为实验组(ACS)组、对照组(sham组)。实验组模型建立成功后再以相应腹内压(20 mmHg)维持0.5、2、4、6 h 4个时间点随机分为4个组,每组6只。每组实验末期,正中剖腹,找到回盲部,切取近端回肠组织,Q-PCR检测TGF-β、cyclin D、CDK-2表达水平,Western blot检测TGF-β、Smad2、磷酸化S-mad2(p-Smad2)蛋白表达水平;此外,如上相同分组共5只大鼠,处理同上,常规HE染色。结果 sham组回肠黏膜上皮细胞结构完整,排列相对整齐;ACS组0.5 h肠黏膜损伤相对较轻,绒毛部分脱落;2 h时肠黏膜明显水肿,肠黏膜上皮间隙增宽,黏膜上皮细胞变性及坏死;4 h和6 h肠绒毛完整性被破坏,绒毛顶端脱落,广泛糜烂,中性粒细胞浸润。ACS组0.5、2、4、6 h组TGF-β表达量较sham组表达降低(P0.05),cyclin D、CDK-2表达量较Sham组升高,差异有统计学意义(P0.05)。ACS组中TGF-β、p-Smad2蛋白表达较sham组降低,差异有统计学意义(P0.05),S-mad2蛋白表达在sham组和ACS组中差异无统计学意义(P0.05)。结论ACS形成后会造成肠黏膜损伤,肠黏膜损+伤后TGF-β信号通路启动一系列分子反应,促进肠黏膜损伤的修复。
[Abstract]:Objective to investigate the role of transforming growth factor- 尾 (TGF- 尾) signaling pathway in the repair of intestinal mucosal injury in rats with abdominal compartment syndrome (ACSS). Methods Thirty SD rats were randomly divided into experimental group and control group. After the establishment of the model, the experimental group was divided into 4 groups with 6 rats in each group. At the end of each experiment, the ileocecal part was found, and the expression level of TGF- 尾 cyclin DnCDK-2 in proximal ileum was detected by Q-PCR. The expression of TGF- 尾 -Smad2, phosphorylS-mad2p-Smad2) protein was detected by Western blot, and 5 rats were divided into the same group as above. Results the structure of ileal mucosal epithelial cells in sham group was intact, the intestinal mucosal injury was relatively mild in sham group at 0.5 h, and the intestinal mucosal mucosal edema was observed at 2 h after partial villi shedding, and the epithelial space of intestinal mucosa widened. The integrity of intestinal villi was destroyed at 4 h and 6 h after denaturation and necrosis of mucosal epithelial cells, the top of villi fell off, and there was extensive erosion. The expression of TGF- 尾 in the neutrophil infiltration group was significantly lower than that in the sham group. The expression of TGF- 尾 in the neutrophil infiltration group was significantly higher than that in the Sham group, and the expression of TGF- 尾 in the ACS group was significantly lower than that in the Sham group. The expression of TGF- 尾 p-Smad2 protein was significantly lower in ACS group than that in sham group. There was no significant difference in P0.05- Smad2 protein expression between sham group and ACS group. Conclusion the formation of ACS may lead to intestinal mucosal injury. TGF- 尾 signaling pathway after intestinal mucosal injury can initiate a series of molecular reactions and promote the repair of intestinal mucosal injury.
【作者单位】：昆明医科大学第一附属医院胃肠外科;昆明医科大学实验动物学部;
【基金】：云南省卫生厅内设机构项目(编号:2014NS124,2014NS122) 云南省卫生科技计划项目(编号:2016NS003)
【分类号】：R-332;R57

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