内脏敏感增高型功能性消化不良脾虚证动物模型的建立

发布时间：2018-04-24 06:46

本文选题：内脏敏感增高型 + 功能性消化不良　；参考：《辽宁中医杂志》2015年11期

【摘要】：目的:探讨内脏敏感增高型功能性消化不良脾虚证动物模型的建立方法并进行模型的评价。方法:运用2%蔗糖溶液灌胃并复合小平台站立法制作内脏敏感增高型功能性消化不良脾虚证动物模型,通过观察大鼠的一般情况,运用ELISA试剂盒检测血清乳酸脱氢酶、血清D-木糖排泄率、血清胃动素的含量,对此模型进行评价。结果:与正常组相比,模型组大鼠的食少、纳呆、腹胀、毛发疏松不泽、神疲乏力等脾虚症状都较其他组重,且持久。在行为学上,碘乙酰胺复合小平台组大鼠的糖水消耗均低于对照组。在指标检测上,模型组大鼠血清D-木糖排泄率、胃动素含量均低于对照组,而乳酸值明显高于对照组(P0.05或P0.01)。结论:通过对模型组大鼠的一般情况和生物学指标的辨证分析,认为内脏敏感增高型功能性消化不良脾虚证动物模型制作成功,此模型达到了"病证结合"的要求。
[Abstract]:Objective: to investigate the establishment and evaluation of the animal model of spleen deficiency syndrome with hypersensitivity of viscera. Methods: the animal model of spleen deficiency syndrome with increased visceral hypersensitivity was made by using 2% sucrose solution by gastric perfusion and combined with small platform station. By observing the general situation of rats, the serum lactate dehydrogenase (LDH) was detected by ELISA kit. Serum D-xylose excretion rate and serum motilin content were evaluated. Results: compared with the normal group, the symptoms of spleen deficiency in the model group were more severe and lasting than those in the other groups, such as less food intake, anorexia, abdominal distension, loose hair, weak spirit and so on. In behavior, the consumption of sugar water in the small platform group was lower than that in the control group. The serum D-xylose excretion rate and motilin content in the model group were lower than those in the control group, but the lactate value was significantly higher than that in the control group (P0.05 or P0.01). Conclusion: by analyzing the general situation and biological index of the model group, it is concluded that the animal model of spleen deficiency syndrome with hypersensitivity of visceral organs is made successfully, and this model meets the requirement of "combination of disease and syndrome".
【作者单位】：北京中医药大学;
【基金】：国家重点基础研究发展计划(973计划)资助项目(2013CB531703)
【分类号】：R-332;R57

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