蓝莓对小鼠急性酒精性脂肪肝TNF-α-R1和SREBP-1c影响的初步研究

发布时间：2018-04-26 20:55

  本文选题：蓝莓 + 酒精性脂肪肝　； 参考：《重庆医科大学学报》2015年03期

【摘要】：目的:观察蓝莓对急性酒精性脂肪肝(alcoholic fatty liver,AFL)小鼠肝组织中肿瘤坏死因子-α-受体1(tumor necrosis factor-α-recepter1,TNF-α-R1)、胆固醇调节元件结合蛋白(sterol regulatory element-binding protein,SREBP)-1c m RNA及蛋白表达的影响。方法:50只C57BL/6雄性小鼠随机分为空白组、模型组、蓝莓汁高剂量组(20.0 g/kg)、蓝莓汁低剂量组(10.0 g/kg)、硫普罗宁组(30 mg/kg),每组10只。除空白组外,其余各组小鼠分别予Lieber-De Carli酒精饲料喂饲,蓝莓汁高、低剂量组及硫普罗宁组同时分别予相应剂量蓝莓汁或硫普罗宁灌胃1次/d,连续15 d,第16天给予1次乙醇灌胃,建立急性AFL动物模型。全自动生化分析仪测定血清丙氨酸氨基转移酶(alanine transaminase,ALT)、天冬氨酸氨基转移酶(aspartate transaminase,AST)、甘油三酯(triglycerides,TG)及胆固醇(cholesterol,TC),酶联免疫吸附法检测血清TNF-α,光镜下观察小鼠肝脏病理学变化,RTPCR、Western blot检测小鼠肝组织中TNF-α-R1、SREBP-1c m RNA及蛋白质表达。结果:蓝莓汁高、低剂量组和硫普罗宁组小鼠血清ALT分别为(67.70±5.86)、(81.66±4.93)、(63.49±3.27)U/L,AST分别为(184.46±14.57)、(214.45±22.03)、(169.16±18.52)U/L,TG分别为(0.88±0.06)、(1.22±0.08)、(0.81±0.09)mmol/L,TC分别为(2.30±0.13)、(2.74±0.10)、(2.21±0.16)mmol/L,TNF分别为(49.14±6.14)、(66.55±5.67)、(44.68±5.39)ng/L,均显著低于模型组(P=0.000或P=0.025),肝细胞病理学改变明显轻于模型组(P=0.000);蓝莓汁高、低剂量组和硫普罗宁组小鼠肝组织TNF-α-R1 m RNA表达分别为100.44±9.51、132.70±8.07、94.02±6.05,蛋白表达分别为0.52±0.04、0.72±0.04、0.49±0.04,SREBP-1c m RNA表达分别为133.52±5.91、174.51±10.62、129.83±9.83,蛋白表达分别为0.26±0.05、0.41±0.03、0.23±0.05,均明显低于模型组(均P=0.000)。结论:蓝莓能改善小鼠急性AFL,可能与下调其肝组织TNF-α-R1与SREBP-1c的表达有关。
[Abstract]:Objective: to observe the effect of blueberry on the expression of TNF- 伪 -R1G, regulatory element-binding protein SSREBPN-1c m RNA and protein in liver of mice with acute alcoholic fatty liver (fatty livera). Methods: TNF- 伪 -receptor 1(tumor necrosis factor- 伪 -receptor 1 (TNF- 伪 -R1) and cholesterol regulatory element binding protein (regulatory element-binding) were detected in mice liver tissues. Methods Fifty male C57BL/6 mice were randomly divided into blank group, model group, high dose group (20.0 g / kg), low dose group (10.0 g / kg), tiopronin group (30 mg / kg), each group (10 rats). In addition to the blank group, the other mice were fed with Lieber-De Carli alcohol feed, the blueberry juice was high, the low-dose group and the tiopronin group were given the corresponding dose of blueberry juice or tiopronin by stomach for one once a day for 15 days, and on the 16th day, the rats were given ethanol. Acute AFL animal model was established. Serum alanine transaminase (Alanine transaminase), aspartate transaminase (aspartate transaminase), triglyceridesy (TGG) and cholesterol (TC) were measured by automatic biochemical analyzer. Serum TNF- 伪 was detected by enzyme-linked immunosorbent assay (Elisa) and pathological changes of mouse liver were observed under light microscope. Blot was used to detect the expression of TNF- 伪 -R1 + SREBP-1c m RNA and protein in liver tissue of mice. 缁撴灉:钃濊帗姹侀珮,浣庡墏閲忕粍鍜岀～鏅�缃楀畞缁勫皬榧犺。�

本文编号：1807608