丙型肝炎病毒F蛋白上调人外周血单个核细胞IL-13的表达

发布时间：2018-04-26 22:15

本文选题：肝炎 + 丙型　；参考：《中华疾病控制杂志》2015年02期

【摘要】：目的探讨丙型肝炎病毒(hepatitis C virus,HCV)F蛋白对白介素13(interluekin-13,IL-13)表达的影响及其相关机制。方法收集慢性HCV1b型患者(chronic hepatitis patient,CHP)的血样,检测F抗体(F antibody,FAb)的阳性率并分成2组(F-Ab(+)CHP、F-Ab(-)CHP);收集健康者血样作为对照组;用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测各组样本血清中IL-13的水平;分离患者外周血单个核细胞(peripheral blood mononuclear cells,PBMC),用不同浓度的F蛋白刺激72 h后,ELISA法检测细胞上清中IL-13的水平,蛋白质免疫印迹(western blotting)法检测细胞中GATA结合蛋白3(GATA binding protein 3,GATA-3)和活化T细胞核因子(nuclear factor of activated T cell,NFAT1)蛋白表达水平。结果 F-Ab(+)CHP组血清IL-13水平高于F-Ab(-)CHP组(P0.001);PBMC经F蛋白体外刺激后,细胞上清中IL-13水平随着F蛋白刺激浓度的增高而增高(x2=13.52,P=0.009);PBMC中GATA-3蛋白表达水平不受F蛋白刺激的影响(均有P0.05),而F蛋白可下调NFAT1蛋白表达。结论在体外培养的PBMC中,F蛋白有上调IL-13水平的作用,而且可能通过下调NFAT1间接发挥作用。
[Abstract]:Objective to investigate the effect of hepatitis C virus hepatitis C virus (HCV) on the expression of interleukin 13 interluekin-13 (IL-13) and its related mechanism. Methods Blood samples of patients with chronic HCV1b type were collected and the positive rate of F antibody F antibody FAbwas detected and divided into 2 groups. The blood samples of healthy subjects were collected as control group, and the serum IL-13 level of each group was detected by enzyme linked immunosorbent assayelia Elisa (enzyme-linked immunosorbent assay (Elisa). Peripheral blood mononuclear cells (PBMC) were isolated from patients with peripheral blood mononuclear cells (PBMC). After 72 h stimulation with different concentrations of F protein, the level of IL-13 in the supernatant was detected by Elisa. Western blotting assay was used to detect the expression of GATA binding protein 3(GATA binding protein 3 (GATA-3) and activated T cell nuclear factor of activated T cell type 1 (NFAT1). Results the level of serum IL-13 in F-AbP group was higher than that in F-Ab(-)CHP group. The level of IL-13 in the supernatant was increased with the increase of the concentration of F protein. The expression of GATA-3 protein was not affected by the stimulation of F protein (P 0.05), but F protein could down-regulate the expression of NFAT1 protein. Conclusion in PBMC cultured in vitro, F protein can up-regulate the level of IL-13, and may play an indirect role by down-regulating NFAT1.
【作者单位】：南京医科大学基础医学院生物化学与分子生物学系;昆明医学院药学院;南京军区疾病预防控制中心疾病预防控制所;中国药科大学生命科学与技术学院;解放军第113医院;宜兴市人民医院检验科;
【基金】：国家自然科学基金(81172724;30972628) 十二五国家科技重大专项(2011ZX10004-902) 江苏省自然科学基金面上项目(BK2010112;BK2011840)
【分类号】：R512.63

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