溃疡性结肠炎患者外周血中IL-32表达水平及其意义

发布时间：2018-04-27 18:03

本文选题：溃疡性结肠炎 + 白细胞介素　；参考：《中国现代医学杂志》2017年29期

【摘要】：目的检测溃疡性结肠炎(UC)患者外周血中白细胞介素32(IL-32)表达水平,分析IL-32表达水平与UC严重程度的关系。方法入组50例UC患者(UC组,根据病情分为轻、中、重度)及30例健康体检者(对照组),分别收集UC组和对照组患者的外周血,用Trizol提取外周血中淋巴细胞总RNA,实时聚合酶链反应检测IL-32 m RNA表达水平;用酶联免疫吸附法检测外周血中IL-32蛋白表达水平,比较两组外周血中IL-32表达水平,以及轻、中、重度UC患者IL-32表达水平。结果 UC患者外周血中IL-32m RNA和蛋白表达水平与对照组比较,差异有统计学意义(P0.05);重度UC患者外周血中IL-32表达水平与轻、中度比较,差异有统计学意义(P0.05)。结论 UC患者外周血中IL-32表达增高,IL-32可能参与UC的发病。
[Abstract]:Objective to detect the expression of interleukin 32 (IL 32) in peripheral blood of patients with ulcerative colitis (UC) and to analyze the relationship between the expression of IL-32 and the severity of UC. Methods Fifty patients with UC were divided into three groups according to their condition: mild, moderate and severe) and 30 healthy persons (control group). The peripheral blood of UC group and control group were collected, respectively. Trizol was used to extract total RNAs from peripheral blood lymphocytes, real-time polymerase chain reaction (PCR) was used to detect the expression level of IL-32 m RNA, and enzyme linked immunosorbent assay (Elisa) was used to detect the expression level of IL-32 protein in peripheral blood. The expression of IL-32 in patients with severe UC. Results the expression level of IL-32m RNA and protein in peripheral blood of UC patients was significantly higher than that of control group (P 0.05), and that of IL-32 in patients with severe UC was significantly higher than that in mild and moderate UC patients (P 0.05). Conclusion the increased expression of IL-32 in peripheral blood of UC patients may be involved in the pathogenesis of UC.
【作者单位】：湖北医药学院附属人民医院胃肠外科;中山大学附属第七医院胃肠外科;
【分类号】：R574.62

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