奥曲肽通过抑制内质网应激减轻肝纤维化大鼠肝损伤

发布时间：2018-04-28 19:15

本文选题：肝损伤 + 奥曲肽　；参考：《实用医学杂志》2017年13期

【摘要】：目的观察奥曲肽(OCT)对肝纤维化(HF)大鼠血清AST、ALT及肝组织Bcl-2、Bax、GRP78、CHOP表达的影响,探讨OCT是否通过抑制内质网应激(ERS)反应减轻大鼠肝损伤。方法将40只SD大鼠随机分为空白(Con)组、模型(Mod)组和低、高剂量OCT治疗(OCT-L、OCT-H)组。除Con组外均采用每周2次皮下注射40%CCl43m L/kg诱导大鼠HF,期间OCT-L、OCT-H组每12小时分别给予OCT 1、10 mg/kg皮下注射。给药8周后处死大鼠,检测大鼠血清AST、ALT含量;HE和Masson染色观察肝组织的病理学改变,Ishak评分系统分析各组肝组织炎症活动度及纤维化程度;Western blot检测肝组织Bcl-2、Bax、GRP78、CHOP蛋白表达。结果与Con组相比,Mod组和OCT治疗组大鼠肝脏病理变化程度、炎症活动度、纤维化程度、血清AST和ALT含量以及Bax、GRP78、CHOP蛋白表达量均显著升高(P0.01),Bcl-2蛋白表达量均明显降低(P0.01)。与Mod组比较,OCT治疗组大鼠肝脏病理变化程度、炎症活动度、纤维化程度、血清AST和ALT含量以及Bax、GRP78、CHOP蛋白表达量显著下降(P0.05),Bcl-2蛋白表达量上升(P0.05),但两OCT治疗组数据差异无统计学意义(P0.05)。结论 OCT可以减轻HF大鼠肝损伤程度,其机制可能与减轻了肝组织的ERS反应有关。
[Abstract]:Objective to observe the effects of octreotide on the expression of alt in serum and liver tissue in rats with hepatic fibrosis, and to explore whether OCT can reduce liver injury by inhibiting endoplasmic reticulum stress (ERS). Methods Forty Sprague-Dawley rats were randomly divided into control group, model control group and low and high dose OCT group. The rats were induced by subcutaneous injection of 40l43m L/kg twice a week except for the Con group, and the OCT-LX OCT-H group was subcutaneously injected with OCT for 10 mg/kg every 12 hours. After 8 weeks of administration, the rats were killed. The serum alt content and the pathological changes of liver tissue were observed by HE and Masson staining. The inflammatory activity and the degree of fibrosis of liver tissue in each group were analyzed. Western blot was used to detect the expression of Bcl-2AXGRP78 chop protein in liver tissue. Results compared with the Con group, the liver pathological changes, the degree of inflammatory activity, the degree of fibrosis, the content of AST and ALT in serum and the protein expression of Bax-GRP78 chop were significantly increased in Mod group and OCT group. Compared with the Mod group, the liver pathological changes, inflammatory activity, fibrosis degree, serum AST and ALT contents and the protein expression of Baxngr GRP78 chop protein in the Oct group increased significantly, but there was no significant difference between the two OCT treatment groups (P 0.05). Conclusion OCT can attenuate the degree of liver injury in HF rats, and its mechanism may be related to the reduction of ERS reaction in liver tissue.
【作者单位】：安徽医科大学第一附属医院肝胆胰外科一病区;
【基金】：安徽省教育厅自然科学基金(编号:KJ2014A117)
【分类号】：R575.2

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