两种不同的酒精摄入方式诱导的小鼠酒精性肝损伤模型比较

发布时间：2018-04-30 20:13

本文选题：酒精性肝损伤 + Lierber-De　；参考：《中国比较医学杂志》2016年06期

【摘要】：目的筛选一种简便、稳定、可靠的酒精性肝损伤模型。方法通过酒精灌胃或给予LierberDe Carli酒精液体饲料8周来建立酒精性肝损伤小鼠模型。实验期间记录小鼠精神状态、摄食量和体重变化,HE染色进行病理学分析,分析血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、γ-谷氨酰转移酶(γ-GT)、碱性磷酸酶(AKP)活性,血清和肝脏总胆固醇(TC)、甘油三酯(TG)含量等肝损伤指标。结果造模8周后,两种方式均导致小鼠肝脏脂质聚集等组织病理学变化,血清ALT、AST、AKP活性、血清和肝脏TG含量均显著提高,提示出现明显肝损伤。酒精灌胃导致小鼠精神状态差,并显著减轻体重,而酒精液体饲料对小鼠精神状态和体重影响小。酒精液体饲料模型肝脏指数和血清TC水平显著增加,而且血清ALT、AST活性、血清和肝脏TG含量及肝脏脂质聚集等组织病理学变化幅度大于酒精灌胃模型,提示前者的肝损伤程度比后者严重。结论 Lieber-De Carli酒精液体饲料模型优于酒精灌胃模型。Lieber-De Carli酒精液体饲料模型相对酒精灌胃模型更适合用于酒精性肝损伤分子机制的研究和防治药物的筛选。
[Abstract]:Objective to screen a simple, stable and reliable alcoholic liver injury model. Methods the model of alcoholic liver injury was established by oral administration of alcohol or LierberDe Carli alcohol liquid feed for 8 weeks. During the experiment, the changes of mental state, food intake and body weight of mice were recorded for pathological analysis by HE staining, and the activities of serum alanine aminotransferase (alt), aspartate aminotransferase (AST), 纬 -glutamyl transferase (纬 -GT), alkaline phosphatase (ALP) were analyzed. Serum and liver total cholesterol (TC), triglyceride (TG) content and other liver injury indexes. Results after 8 weeks of modeling, both of the two methods resulted in histopathological changes such as lipid aggregation in the liver of mice. The activity of AKP in serum of alt ASTN and the content of TG in serum and liver were significantly increased, indicating that there was obvious liver injury. Alcohol administration resulted in poor mental state and significant weight loss in mice, while alcohol liquid diet had little effect on mental state and body weight of mice. The liver index and serum TC level of alcohol liquid feed model were significantly increased, and the activity of alt AST, TG content of serum and liver, liver lipid aggregation and other histopathological changes were larger than those of alcohol gastric perfusion model. These results suggest that the liver injury of the former is more serious than that of the latter. Conclusion the Lieber-De Carli alcohol liquid feed model is better than the alcohol perfusion model. Lieber-De Carli alcohol feed model is more suitable for the study of the molecular mechanism of alcoholic liver injury and the screening of preventive and therapeutic drugs.
【作者单位】：华南农业大学食品学院;广东省农业科学院蚕业与农产品加工研究所/农业部功能食品重点实验室/广东省农产品加工重点实验室;深圳职业技术学院;
【基金】：国家自然科学基金-青年科学基金项目(NSFC 31501478) 广东省自然科学基金博士启动项目(2014A030310328) 深圳市科技计划基础研究项目(JCYJ20140901162541286) 广东省省级科技计划项目(2016B070701012)
【分类号】：R575;R-332

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