缺血后处理对梗阻性黄疸肾缺血再灌注损伤大鼠血清TNF-α水平的影响

发布时间：2018-05-02 00:22

本文选题：梗阻性黄疸 + 缺血后处理　；参考：《山东医药》2015年05期

【摘要】：目的探讨缺血后处理对梗阻性黄疸肾缺血再灌注损伤大鼠血清TNF-α水平的影响。方法 60只SD大鼠随机分成梗阻性黄疸对照组(S组)、梗阻性黄疸肾缺血再灌注组(I/R组)、梗阻性黄疸肾缺血再灌注缺血后处理组(IPO组),每组20只。根据缺血后再灌注时间点各组又分为再灌注0 h(T0)、1 h(T1)、3 h(T2)、6 h(T3)4个亚组各5例。检测再灌注后的各时点血清中尿素氮(BUN)、肌酐(Cr)、TNF-α水平,观察肾组织的病理改变。结果 I/R组和IPO组血清BUN、Cr、TNF-α水平均高于S组(P均0.05),IPO组T2时间点血清Cr、BUN、TNF-α水平均低于I/R组(P均0.05)。结论缺血后处理可以下调大鼠血清TNF-α水平,从而减轻梗阻性黄疸大鼠肾缺血再灌注损伤,其机制可能与抑制炎症反应有关。
[Abstract]:Objective to investigate the effect of post-ischemic treatment on serum TNF- 伪 level in rats with renal ischemia reperfusion injury caused by obstructive jaundice. Methods Sixty Sprague-Dawley rats were randomly divided into two groups: obstructive jaundice control group (S group), obstructive jaundice renal ischemia reperfusion group (I / R group), obstructive jaundice renal ischemia reperfusion group (IPO group) with 20 rats in each group. According to the time points of reperfusion after ischemia, each group was divided into 4 subgroups (5 cases each). The levels of bun, Cr, TNF- 伪 in serum at different time points after reperfusion were detected, and the pathological changes of renal tissue were observed. Results the serum levels of BUNA TNF- 伪 in group I / R and group IPO were higher than those in group S (P = 0.05) and those in group I / R were lower than those in group I / R (P < 0.05). Conclusion the level of TNF- 伪 in serum of rats can be down-regulated by post-ischemic treatment, and the renal ischemia-reperfusion injury can be alleviated in rats with obstructive jaundice. The mechanism may be related to the inhibition of inflammation.
【作者单位】：中山大学附属第五医院;
【基金】：珠海市科研计划基金项目(2014D0401990018)
【分类号】：R575

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