肝硬化患者肠道粘膜通透性及其与Child-Pugh分级相关性的研究

发布时间：2018-05-03 14:00

本文选题：肝硬化 + 研究　；参考：《中华疾病控制杂志》2015年04期

【摘要】：目的了解肝硬化患者肠道粘膜通透性的改变并探讨其与Child-Pugh分级之间的相关性。方法纳入66例肝硬化患者,并选择40例健康体检者作为对照,采用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测血二胺氧化酶(diamine oxidase,DAO)、D-乳酸水平,采用高效液相色谱法测定尿乳果糖/甘露醇(lactulose/mannitol,L/M)排泄率比来评价肠道粘膜通透性。结果肝硬化患者Child-Pugh分级各组的血DAO、D-乳酸水平及尿L/M排泄率比均明显高于对照组,差异有统计学意义(均有P0.05)。血DAO水平与尿L/M排泄率比、血D-乳酸水平与尿L/M排泄率比均呈线性相关(r=0.518、r=0.527,均有P0.05)。血DAO水平、血D-乳酸水平及尿L/M排泄率比与Child-Pugh评分均呈正相关(rs=0.705、rs=0.791、rs=0.851,均有P0.05)。结论血DAO、D-乳酸水平及尿L/M排泄率比三种检测指标能准确反映肠粘膜通透性的变化。肝硬化患者的肠粘膜通透性较对照组明显增高,且与Child-Pugh评分呈正相关,肠粘膜通透性的检测对肝硬化患者的治疗有一定的临床价值。
[Abstract]:Objective to understand the changes of intestinal mucosal permeability in patients with liver cirrhosis and to explore the correlation between Child-Pugh classification and methods. Methods the methods were included in 66 patients with liver cirrhosis, and 40 healthy subjects were selected as control, and the blood two amine oxidase (diamine oxidase, DAO) was detected by enzyme-linked immunosorbent assay (ELISA), D- The level of lactate (lactulose/mannitol, L/M) excretion rate was measured by high performance liquid chromatography (HPLC) to evaluate the permeability of intestinal mucosa. Results the blood DAO, D- lactate level and urine L/M excretion ratio of Child-Pugh groups in the patients with cirrhosis were significantly higher than those in the control group. The difference was statistically significant (P0.05). Blood DAO level. Compared with urine L/M excretion rate, the level of blood D- lactate and urine L/M excretion rate were linearly correlated (r=0.518, r=0.527, P0.05). Blood DAO level, serum D- lactate level and urinary L/M excretion rate were positively correlated with Child-Pugh score (rs=0.705, rs=0.791, all). The standard can accurately reflect the changes of intestinal permeability. The intestinal mucosal permeability of the patients with liver cirrhosis is significantly higher than that of the control group, and it has a positive correlation with the Child-Pugh score. The detection of intestinal mucosal permeability has certain clinical value for the treatment of patients with liver cirrhosis.

【作者单位】：安徽医科大学第一附属医院消化内科;
【分类号】：R575.2

【参考文献】