急性胰腺炎中Gli1介导的炎性细胞因子的表达变化及意义

发布时间：2018-05-06 08:17

本文选题：Gli + 远隔组织　；参考：《中国免疫学杂志》2017年07期

【摘要】：目的:探讨在急性胰腺炎中通过调控Gli1信号后观察炎性细胞因子IL-6的表达变化及意义。方法:本实验将C57小鼠随机分为3组:对照组、模型组、抑制组。雨蛙素腹腔注射诱导急性胰腺炎模型,HE染色、检测淀粉酶水平证明模型造模成功,用RT-q PCR、Western blot检测Gli1在胰腺、肝、小肠、肺、肾组织的表达。用ELISA方法检测炎性细胞因子IL-6。结果:雨蛙素腹腔注射诱导急性胰腺炎模型,HE染色、检测淀粉酶水平证明模型造模成功,用RT-q PCR、Western blot检测Gli1在胰腺、肝、小肠、肺、肾组织的表达。用ELISA方法检测炎性细胞因子IL-6。与对照组相比较,雨蛙素注射后的模型组各个组织(胰腺、肝、小肠、肺、肾组织)Gli1表达较对照组升高,再用Gant61(Gli特异性抑制剂)抑制Gli1后可见抑制组IL-6表达较模型组升高。结论:在急性胰腺炎症过程中,Gli1可能参与了AP过程中远隔组织损伤和修复过程,且gli1可能通过调控其下游的炎性细胞因子IL-6而发挥保护作用。
[Abstract]:Aim: to investigate the expression and significance of inflammatory cytokine IL-6 in acute pancreatitis after regulating Gli1 signal. Methods: C57 mice were randomly divided into three groups: control group, model group and inhibition group. The rat model of acute pancreatitis induced by intraperitoneal injection was stained with HE and the amylase level was detected. The expression of Gli1 in pancreas, liver, small intestine, lung and kidney was detected by RT-q PCR Western blot. IL-6 was detected by ELISA. Results: the rat model of acute pancreatitis induced by intraperitoneal injection of Rana farinae was stained with HE, the amylase level was detected and the expression of Gli1 in pancreas, liver, small intestine, lung and kidney was detected by RT-q PCR Western blot. IL-6 was detected by ELISA. Compared with the control group, the expression of Gli1 in all tissues (pancreas, liver, small intestine, lung, kidney) of the model group (pancreas, liver, small intestine, lung and kidney) was higher than that in the control group, and the expression of IL-6 in the inhibition group was higher than that in the model group after inhibiting Gli1 with Gant61(Gli specific inhibitor. Conclusion: Gli1 may be involved in the injury and repair of distant tissue in the process of AP, and gli1 may play a protective role by regulating the inflammatory cytokine IL-6 downstream of AP.
【作者单位】：西南医科大学附属医院甲状腺血管外科;
【基金】：国家自然科学基金资助项目(No.81100272)
【分类号】：R576

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