非酒精性脂肪性肝病患者肝组织水通道蛋白表达差异与多烯磷脂酰胆碱疗效相关性分析

发布时间：2018-05-07 05:02

本文选题：水通道蛋白 + 多烯磷脂酰胆碱　；参考：《第三军医大学学报》2017年18期

【摘要】：目的分析非酒精性脂肪性肝病(nonalcoholic fatty liver disease,NAFLD)患者肝组织水通道蛋白(aquaporin,AQPs)3、7、9表达水平对多烯磷脂酰胆碱(plyene posphatidyl coline,PPC)疗效的影响。方法分析2014-2016年本院收治的NAFLD患者87例,按病程分组:肝硬化组(n=30),肝纤维化组(n=24),肝炎组(n=33);超声疗效评估分组:有效组和无效组。随访不同病程的NAFLD患者PPC治疗前后的肝功能酶变化,按照PPC疗效进行分组,比较患者肝组织AQP3、7、9蛋白的表达差异,并进一步分析PPC疗效与AQPs表达水平的相关性。结果 PPC对NAFLD患者治疗整体有效率35.63%,PPC对NAFLD疗效与病程严重程度无关,但与超声的评分密切相关,表现为按照超声评分有效和无效分组的不同病程组患者的谷丙转氨酶(ALT)在PPC治疗后,在每种病程患者中差异有统计学意义,且ALT在PPC治疗后,其变化程度与超声的变化程度呈正相关,肝硬化组(R=0.780,P0.01),肝纤维化组(R=0.879,P0.01),肝炎组最高(R=0.900,P0.01)。PPC治疗NAFLD效果与患者初始的肝组织AQPs的表达存在相关性:NAFLD患者肝组织AQP7、9表达量与肝功能酶的变化量呈正相关(AQP9:R=0.972,AQP7:R=0.537),而AQP3的表达水平与肝功能酶的变化量呈负相关(R=-0.881);AQP7、9表达量与超声评分的变化量呈正相关(AQP9:R=0.763,AQP7:R=0.311),而AQP3的表达水平与肝功能酶的变化量呈负相关(R=-0.795)。结论多烯磷脂酰胆碱对非酒精性脂肪性肝病的疗效与水通道蛋白表达差异密切相关,提示AQPs表达水平可能影响PPC疗效。
[Abstract]:Objective to investigate the effect of aquaporinus aquaporin (aquaporin) on the therapeutic effect of polyphosphatidylcholine (PPC) plyene posphatidyl in the liver tissue of patients with nonalcoholic fatty liver disease (NAF LDD). Methods Eighty-seven patients with NAFLD from 2014-2016 were divided into three groups: liver cirrhosis group (n = 30), hepatic fibrosis group (n = 24), hepatitis group (n = 33) and ultrasonic evaluation group: effective group (n = 30) and ineffective group (n = 30). Liver function enzyme changes of NAFLD patients with different course of disease were followed up before and after PPC treatment. According to the effect of PPC, the difference of expression of AQP3O7P9 protein in liver tissue was compared, and the correlation between PPC efficacy and AQPs expression level was further analyzed. Results the overall effective rate of PPC in the treatment of NAFLD patients was 35.63%. The curative effect of NAFLD was not related to the severity of the course of disease, but was closely related to the score of ultrasound. The results showed that there were significant differences in alt between the patients with different course of disease and the patients with different course of disease according to the effective and ineffective ultrasound scores after PPC treatment, and the difference of ALT after PPC treatment was statistically significant in each course of the patients, and there was no significant difference between the two groups after PPC treatment. There was a positive correlation between the degree of change and the degree of ultrasound. There was a positive correlation between the expression of AQP7O9 in liver tissues of patients with liver cirrhosis and the changes of liver function enzymes in liver tissues of patients with liver fibrosis and the expression of AQPs in liver tissues of patients with liver fibrosis, while the expression of AQP3 was water in the expression of AQP3 in the liver tissues of the patients with liver fibrosis (P < 0.05) and liver fibrosis group (n = 0.879). The highest effect of RPPC in the treatment of NAFLD was found in the patients with liver cirrhosis. There was a positive correlation between the expression of AQP7O9 in liver tissues and the changes of liver function enzymes in the patients with liver fibrosis, while the expression of AQP3 in the liver tissues was positively correlated with the expression of AQPs in the liver tissues of the patients with liver fibrosis. There was a negative correlation between the expression of AQP7H9 and the changes of liver function enzymes. There was a positive correlation between the expression of AQP9: RP9: AQP7: R7: R3, and a negative correlation between the expression level of AQP3 and the changes of liver function enzymes. Conclusion the therapeutic effect of polyenylphosphatidylcholine on non-alcoholic fatty liver disease is closely related to the difference of aquaporin expression, suggesting that the expression level of AQPs may affect the curative effect of PPC.
【作者单位】：成都军区总医院消化内科;成都军区总医院检验科;
【基金】：四川省卫生厅资助项目(120572)~~
【分类号】：R575.5

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