急性肝损伤大鼠肝组织HSP60的变化及意义

发布时间：2018-05-10 05:17

本文选题：大鼠 + 肝损伤　；参考：《中华灾害救援医学》2017年02期

【摘要】：目的观察大鼠急性肝损伤过程中热休克蛋白60(heat shock protein 60,HSP60)的变化及其与肝损伤的相关性。方法 126只清洁级SD大鼠,随机分为空白组(n=42)、非肝损伤组(n=42)和肝损伤组(n=42)。空白组不作任何处理;非肝损伤组大鼠进行左腿大腿骨折处理,肝损伤组用改良型BIM-Ⅳ(biological impact machine-Ⅳ)生物撞击机撞击诱导急性肝损伤。各组处理后于6 h、12 h、1 d、2 d、3 d、5 d、7 d取血液检测丙氨酸转氨酶(alanine aminotransferase,ALT)、天冬氨酸转氨酶(aspartate transaminase,AST)水平;之后杀鼠取肝,苏木精—伊红染色法(hematoxylin-eosin,HE)染色观察肝组织病理变化;免疫组化染色检测HSP60蛋白表达。结果 (1)空白组与非肝损伤组在上述各时间段的ALT、AST表达水平差异无统计学意义(P0.05);与空白组及非肝损伤组相比,肝损伤组于6 h、12 h、1 d、2 d、3 d、5 d、7 d血清ALT、AST含量显著增高(P0.05),且在6 h显著升高[(104.17±4.07)U/L,(271.00±15.84)U/L],12 h达到顶峰[(424.50±10.84)U/L,(787.70±32.68)U/L],之后不断下降,在3 d时恢复正常;(2)HE染色示,肝损伤组肝小叶结构排列紊乱,气球样变明显,在12 h最为明显;(3)免疫组化HSP60染色,肝损伤组织在6 h开始显著高于空白组和非肝损伤组,12 h达到顶峰,1~3 d持续高于空白组和非肝损伤组,5 d时恢复正常水平;(4)HSP60蛋白表达与血清中ALT(r=0.9064,P0.05)、AST(r=0.9294,P0.05)表达呈正相关。结论大鼠急性肝损伤过程中HSP60的表达量有明显的动态变化规律,与大鼠肝脏损伤程度变化一致。
[Abstract]:Objective To observe the changes of heat shock protein 60 (heat shock protein 60, HSP60) and the correlation with liver injury during acute liver injury in rats. Methods 126 clean SD rats were randomly divided into blank group (n=42), non liver injury group (n=42) and liver injury group (n=42). No treatment was done in the empty white group. The left leg and thigh of the rats in the non liver injury group were carried out in the left leg and thigh. Acute liver injury was induced by a modified BIM- IV (biological impact machine- IV) biological impact machine for the treatment of fracture. After treatment, each group was treated with 6 h, 12 h, 1 D, 2 D, 3 D, 5 d, 7 d, and the level of alanine aminotransferase (alanine), and aspartate aminotransferase. The liver, hematoxylin and eosin staining (hematoxylin-eosin, HE) staining was used to observe the pathological changes of liver tissue, and the expression of HSP60 protein was detected by immunohistochemical staining. Results (1) there was no significant difference in the expression of ALT and AST in the blank group and the non liver injury group at the above time segments (P0.05). Compared with the blank group and the non liver injury group, the liver injury group was 6 h, 12 H, 1 D, 2 D, 3 D, 5 d, and 7 d serum ALT, AST content increased significantly (P0.05), and the 6 h significantly increased [(104.17 + 4.07) U/L, (271 + 15.84) U/L], (424.50 + 10.84) reached the peak [(424.50 + 10.84)]. (3) (3) immuno histochemical HSP60 staining, liver injury tissue at 6 h began to significantly higher than the blank group and non liver injury group, 12 h reached the peak, 1~3 D continued to be higher than the blank group and non liver injury group, 5 d to restore normal level; (4) the expression of HSP60 protein and serum ALT (r= 0.9064, P0.05), AST (r=0.9294,) expression is positive. The expression of HSP60 in the process of hepatic injury has a significant dynamic change, which is consistent with the degree of liver injury in rats.

【作者单位】：新疆维吾尔自治区石河子大学医学院第一附属医院急诊科;
【基金】：国家自然科学基金(81560312)
【分类号】：R575.3

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