TNFSF14及其受体LTβR和HVEM在病毒肝炎中的作用

发布时间：2018-05-10 07:48

本文选题：TNFSF + LTβR　；参考：《中国免疫学杂志》2015年12期

【摘要】：目的:探讨TNFSF14(即LIGHT)及其受体LTβR和HVEM在暴发性病毒肝炎中的作用。方法:MHV-3感染易感小鼠建立暴发性病毒肝炎模型,通过HE染色和血清ALT水平的测定比较了LIGHT KO和WT两组小鼠的肝损情况;定量PCR技术检测了MHV-3感染C57BL/6小鼠后各时相点(0 h、12 h、24 h、48 h、72 h)肝脏和脾脏组织HVEM和LTβR的mRNA水平。流式细胞术检测了临床病毒性重症肝炎病人PBMC中HVEM和LTβR的表达情况。结果:MHV-3感染72 h后,WT小鼠的肝脏出现明显坏死灶,而LIGHT KO小鼠的肝脏则未见异常;LIGHT KO小鼠血清中的ALT浓度也显著低于WT组(P0.01)。MHV-3感染48 h后,C57BL/6小鼠脾脏中HVEM及LTβR的mRNA水平均有显著升高(P0.05);肝脏中LTβR表达在12 h时就有显著上调(P0.01)。与之一致的是,临床重症肝炎病人PBMC中HVEM和LTβR的表达较正常人均有明显升高。结论:TNFSF14可能通过与其受体LTβR和HVEM的相互作用在病毒诱发的暴发性肝炎中扮演重要角色。
[Abstract]:Aim: to investigate the role of TNFSF14 and its receptors LT 尾 R and HVEM in fulminant viral hepatitis. Methods the fulminant viral hepatitis model was established in susceptible mice infected with MHV-3. The liver damage of LIGHT KO and WT groups were compared by HE staining and serum ALT level. The mRNA levels of HVEM and LT 尾 R in liver and spleen tissues of C57BL/6 mice infected with MHV-3 were determined by quantitative PCR. The expression of HVEM and LT 尾 R in PBMC of patients with severe viral hepatitis was detected by flow cytometry. Results 72 hours after infection, liver necrosis was observed in the mice with WMHV-3 infection. However, the serum ALT concentration of LIGHT KO mice was significantly lower than that of WT group P0.01U. MHV-3. The mRNA levels of HVEM and LT 尾 R in spleen of C57BL / 6 mice were significantly higher than those of WT group 48 h after infection, and the expression of LT 尾 R increased significantly at 12 h. Similarly, the expression of HVEM and LT 尾 R in PBMC of patients with severe hepatitis was significantly higher than that in normal controls. Conclusion: TNFSF14 may play an important role in viral induced fulminant hepatitis by interacting with its receptor LT 尾 R and HVEM.
【作者单位】：第三军医大学基础部免疫学教研室;第三军医大学附属大坪医院泌尿外科;重庆医科大学附属儿童医院儿科研究所流式细胞室;
【基金】：国家自然科学基金面上项目(31270929) 重庆市自然科学基金面上项目(cstc2013jcyj A10129)资助
【分类号】：R512.6

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