血管活性肠肽对便秘大鼠排便及结肠组织中VIP-cAMP-PKA-AQP3信号通路的影响

发布时间：2018-05-10 09:09

本文选题：便秘 + 血管活性肠肽　；参考：《中南大学学报(医学版)》2016年11期

【摘要】：目的:观察血管活性肠肽(vasoactive intestinal peptide,VIP)对便秘大鼠肠道水液代谢、环磷酸腺苷-蛋白激酶A信号通路(cyclic AMP protein kinase A signaling pathway,c AMP-PKA)和水通道蛋白3(water channel protein 3,AQP3)的影响,探讨VIP治疗便秘的作用及机制。方法:45只健康成年Sprague-Dawley大鼠随机分为空白对照组、模型组、模型+VIP组。给药4周后,墨汁灌胃法检测大鼠首粒黑便排出时间;根据大鼠粪便干湿重计算粪便含水率;HE染色观察各组大鼠结肠组织形态学变化;Western印迹检测各组大鼠结肠组织中VIP和AQP3蛋白表达水平;定量即时聚合酶链锁反应(quantitative real time polymerase chain reaction,q PCR)检测各组大鼠结肠组织中c AMP,PKA和AQP3 m RNA的表达水平。结果:与空白对照组比较,模型组大鼠首粒黑便出现时间延长,粪便含水率明显减少(均P0.01);结肠黏膜上皮部分破坏,杯状细胞体积减小,数量明显减少;结肠组织中VIP和AQP3蛋白含量明显减少,AQP3,c AMP和PKA m RNA相对表达水平均有所降低(均P0.05)。与模型组比较,模型+VIP组大鼠首粒黑便出现时间缩短,粪便含水率明显增加(均P0.05);结肠黏膜上皮完整性明显改善,杯状细胞体积增大,数量增多;结肠组织中VIP和AQP3蛋白含量增多,CAMP,PKA和AQP3 m RNA相对表达水平升高(均P0.05)。结论:VIP静脉注射能够调节肠道水液代谢,改善大鼠便秘症状,其机制可能与调节VIP-c AMP-PKA-AQP3信号通路有关。
[Abstract]:Aim: to investigate the effects of vasoactive intestinal peptide (VIPP) on intestinal water metabolism, cyclic AMP protein kinase A signaling pathwayc AMP-PKA and aquaporin 3(water channel protein 3 aquaporin (AQP3) in rats with constipation, and to explore the mechanism of VIP in the treatment of constipation. Methods 45 healthy adult Sprague-Dawley rats were randomly divided into control group, model group and model VIP group. After 4 weeks of administration, the time of excretion of the first black stool was detected by the method of Chinese ink gavage. The morphological changes of colonic tissue were observed by HE staining. The expression of VIP and AQP3 protein in colonic tissue of rats in each group was detected by Western blot. Quantitative real time polymerase chain reactionQ PCR was used to detect the expression of c-AMPP-PKA and AQP3 m RNA in colonic tissues of rats in each group. Results: compared with the blank control group, the appearance time of the first black stool in the model group was prolonged, and the fecal moisture content was significantly decreased (all P 0.01), the colonic mucosal epithelium was partially destroyed, the goblet cell volume was decreased and the number of goblet cells was significantly decreased. The protein content of VIP and AQP3 in colonic tissue was significantly decreased, and the relative expression of AQP3C AMP and PKA m RNA were all decreased (P0.05). Compared with the model group, the appearance time of the first black stool was shortened and the fecal moisture content was significantly increased in the model VIP group (all P 0.05), the integrity of colonic mucosal epithelium was improved significantly, the goblet cell volume was increased and the number of goblet cells was increased. The protein content of VIP and AQP3 increased and the relative expression of AQP3 m RNA and PKA increased in colon tissue (P0.05). Conclusion the control of intestinal fluid metabolism and the improvement of constipation symptoms may be related to the regulation of VIP-c AMP-PKA-AQP3 signaling pathway.
【作者单位】：陕西中医药大学基础医学院;陕西中医药大学人事处;陕西中医药大学协同创新中心;
【基金】：国家自然科学基金(81273663) 陕西省教育厅项目(320104-203010021)~~
【分类号】：R574.62

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