美沙拉秦、蒙脱石散和酪酸梭菌对溃疡性结肠炎大鼠血细胞因子的影响

发布时间：2018-05-12 07:06

本文选题：美沙拉秦 + 蒙脱石散　；参考：《中国免疫学杂志》2015年02期

【摘要】：目的:研究美沙拉秦、蒙脱石散、酪酸梭菌对2,4,6三硝基苯磺酸(TNBS)/乙醇法诱导的溃疡性结肠炎大鼠IL-17、IL-23、TGF-β1、IFN-γ的影响。方法:将85只大鼠随机分为模型组、美沙拉秦组、蒙脱石散组、酪酸梭菌组、美沙拉秦联合蒙脱石散组5组,每组15只;正常大鼠为空白对照组10只,一共6组。通过TNBS/乙醇法建立溃疡性结肠炎的大鼠模型。按大鼠体表面积折算药量,分别给予相应剂量生理盐水、美沙拉秦、蒙脱石散、酪酸梭菌、美沙拉秦联合蒙脱石散治疗。12 d后处死全部大鼠,收集大鼠的血标本及结肠标本。采用ELISA法测定血清中IL-17、IL-23、TGF-β1、IFN-γ的含量,分别对比6组细胞因子的水平。结果:(1)通过TNBS/乙醇法建立大鼠模型,HE染色病理切片发现大鼠结肠远端炎症明显。(2)模型组、美沙拉秦组、蒙脱石散组、酪酸梭菌组、美沙拉秦联合蒙脱石散组较空白对照组的血清IL-17、IL-23和IFN-γ含量均升高,血清TGF-β1含量均降低,差异有统计学意义(P0.05)。(3)与模型组比较,蒙脱石散组、酪酸梭菌组、美沙拉秦组的血清IL-17、IL-23和IFN-γ含量均降低,血清TGF-β1含量均升高,差异有统计学意义(P0.05)。(4)美沙拉秦、蒙脱石散、酪酸梭菌和美沙拉秦联合蒙脱石散的治疗效果比较,美沙拉秦和美沙拉秦联合蒙脱石散疗效较好,差异有统计学意义(P0.05),酪酸梭菌与蒙脱石散疗效差异不明显。结论:(1)从症状体征及病理学检查表明:TNBS/乙醇法成功建立了大鼠溃疡性结肠炎模型。(2)在TNBS/乙醇法诱导的实验性溃疡性结肠炎大鼠血清中IL-17、IL-23、IFN-γ表达增高,与炎症呈正相关;结肠炎大鼠血清中TGF-β1表达降低,与炎症呈负相关。(3)美沙拉秦、蒙脱石散、酪酸梭菌、美沙拉秦联合蒙脱石散可能通过下调模型大鼠促炎因子IL-17、IL-23和调节因子IFN-γ的分泌,上调抗炎因子TGF-β1的分泌,缓解大鼠炎症症状,利于大鼠溃疡性结肠炎的修复。
[Abstract]:Aim: to study the effects of Mesalazin, montmorillonite powder and Clostridium caseate on IL-17 and IL-23 TGF- 尾 1 IFN- 纬 in rats with ulcerative colitis induced by TNBSU / ethanol. Methods: Eighty-five rats were randomly divided into three groups: model group, Mesalazin group, montmorillonite powder group, Clostridium caseinate group, Mesalazin combined with montmorillonite powder group with 15 rats in each group, and normal rats as blank control group (n = 10). The rat model of ulcerative colitis was established by TNBS/ ethanol method. The rats were given corresponding doses of normal saline, mesalazin, montmorillonite powder, Clostridium caseinate, Mesalazin combined with montmorillonite powder. All rats were killed after 12 days of treatment. Blood samples and colon specimens of the rats were collected. The serum levels of IL-17, IL-23, TGF- 尾 1, IFN- 纬 and cytokines were determined by ELISA method. Results (1) the rat model was established by TNBS/ ethanol method. The pathological sections of HE staining showed that the inflammation of distal colon was obvious in rats. (2) Model group, Mesalazin group, montmorillonite powder group, Clostridium caseinate group. Compared with the model group, the serum levels of IL-17, IL-23 and IFN- 纬 increased and the levels of serum TGF- 尾 1 decreased in the Mesalazin combined with montmorillonite powder group. The difference was statistically significant compared with the model group, the montmorillonite powder group and the Clostridium caseinate group. The serum levels of IL-17, IL-23 and IFN- 纬 decreased, and the levels of serum TGF- 尾 1 increased in the melalazin group. The difference was statistically significant (P 0.05. 05. 4) the therapeutic effects of Mesalazin, montmorillonite powder, Clostridium caseinate and melarazin combined with montmorillonite powder were compared. The curative effect of Mesalazin and Mesalazin combined with montmorillonite powder was better, the difference was statistically significant (P 0.05), but there was no significant difference between Clostridium caseinate and montmorillonite powder. Conclusion (1) the rat model of ulcerative colitis was successfully established by the TNBS/ ethanol method. (1) the expression of IL-17, IL-23, IFN- 纬 in the serum of rats with experimental ulcerative colitis induced by TNBS/ ethanol was increased, and the expression of IL-17 IL-23 IFN- 纬 was positively correlated with inflammation. The expression of TGF- 尾 1 in the serum of colitis rats was decreased, and negatively correlated with inflammation. The expression of Mesalazin, montmorillonite powder, Clostridium caseinate and Mesalazine combined with montmorillonite powder may be down-regulated by down-regulating the secretion of IL-17, IL-23 and IFN- 纬, the inflammatory factor IL-17 and the regulatory factor IFN- 纬 in rats with colitis. Upregulate the secretion of anti-inflammatory factor TGF- 尾 1, relieve the inflammatory symptoms of rats, and facilitate the repair of ulcerative colitis in rats.
【作者单位】：大理白族自治州人民医院儿科;昆明医科大学第一附属医院儿科;昆明医科大学附属儿童医院;
【基金】：益普生腹泻基金资助项目(IDF-2012-01)
【分类号】：R574.62

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