肝硬化及肝细胞癌患者肠道微生态组成和结构的初步分析

发布时间：2018-05-14 00:17

  本文选题：肝硬化 + 癌　； 参考：《临床肝胆病杂志》2017年09期

【摘要】：目的比较分析肝硬化和肝硬化基础上发生肝细胞癌(HCC)的患者远端肠道菌群的差异,探讨肝硬化患者发生HCC与肠道菌群变化的关系。方法收集2015年12月-2016年5月天津市第三中心医院肝内科住院的慢性肝病患者35例,包括20例肝硬化患者(肝硬化组)和15例肝硬化合并HCC患者(HCC组)。收集患者粪便标本,提取其肠道细菌总DNA,采用Roche 454测序技术对16S r DNA V3-V6可变区测序,并进行生物信息分析(物种分类、丰度分析及多样性分析)。计量资料组间比较采用t检验,计数资料组间比较采用Mann-Whitney U检验。结果 20个肝硬化患者样品平均OTU为306.50±163.76,15个HCC患者样品平均OTU为357.24±168.85,2组患者OTU及alpha多样性指数相比,差异没有统计学意义(P值均0.05)。粪便菌群序列包括的菌门主要有放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)和变形菌门(Proteobacteria)。属种定量及组成分析发现,肝硬化组和HCC组患者肠道内多种细菌的相对丰度差异显著,其中肝硬化组肠道菌群与HCC组比较,放线菌门(0.21%vs 0.06%,U=89.000,P=0.043)、双歧杆菌属(0.16%vs 0.04%,U=90.000,P=0.046)和梭菌属(0.13%vs 0.08%,U=90.000,P=0.046)所占比例显著升高,理研菌科(0.58%vs 2.30%,U=82.000,P=0.023)和Christenellaceae科(0.01%vs0.08%,U=84.500,P=0.028)所占比例显著降低。结论与单纯肝硬化患者相比,肝硬化基础上发生HCC者肠道菌群的组成有显著差异,但这些差异与肝硬化基础上发生HCC之间相互作用的具体机制尚不明确。
[Abstract]:Objective to investigate the relationship between HCC and intestinal flora in patients with cirrhosis and cirrhosis. Methods from December 2015 to May 2016, 35 patients with chronic liver disease, including 20 patients with liver cirrhosis and 15 patients with HCC, were enrolled in Department of Hepatology, third Center Hospital of Tianjin. The fecal samples of patients were collected and the total intestinal bacterial DNA was extracted. The 16s r DNA V3-V6 variable region was sequenced by Roche 454 sequencing technique, and the biological information was analyzed (species classification, abundance analysis and diversity analysis). T test was used to compare measurement data and Mann-Whitney U test was used to compare counting data. Results the average OTU of 20 patients with liver cirrhosis was 306.50 卤163.76, and the average OTU of 15 patients with HCC was 357.24 卤168.85. There was no significant difference in the diversity index of OTU and alpha between the two groups (P < 0.05). The sequence of faecal bacteria includes Actinobacteria, Bacteroidetesa, Firmicutesand Proteobacteria. Quantitative analysis and composition analysis showed that the relative abundance of many bacteria in the intestine of patients with cirrhosis and HCC was significantly different, and the intestinal flora of cirrhosis group was higher than that of HCC group. The proportions of 0.21 vs 0.06 U89.000 P0.043, 0.16 vs 0.04 000 of Bifidobacterium and 0.13% of Clostridium 0.13 vs 0.080.000 P0.046) increased significantly, and the percentages of 0.58 vs 2.30% of Rhizoma 82.000 P0.023) and 0.01vs0.084.500 (P0.028) decreased significantly. Conclusion there are significant differences in the composition of intestinal flora in patients with HCC on the basis of cirrhosis as compared with those in patients with cirrhosis alone, but the specific mechanism of the interaction between these differences and HCC on the basis of cirrhosis is not clear.
【作者单位】： 天津医科大学三中心临床学院天津市第三中心医院肝内科天津市人工细胞重点实验室天津市肝胆疾病研究所;天津市河东区口腔医院;
【基金】：国家科技重大专项(2016ZX10002008-07) 天津市科技计划项目(13RCGFSY19200)
【分类号】：R575.2;R735.7
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