Keap1、Nrf2和HO-1在大鼠应激性胃溃疡中的表达

发布时间：2018-05-15 22:25

本文选题：应激性胃溃疡 + Keap-　；参考：《江苏农业科学》2016年12期

【摘要】：探讨Kelch样环氧氯丙烷相关蛋白-1(Kelch-like epichlorohydrin-associated protein 1,Keap1)/核因子E2相关因子2(nuclear factor-E2-related factor 2,Nrf2)/血红素氧合酶-1(heme oxygenase 1,HO-1)信号通路在应激性胃溃疡发生及发展中的作用。建立大鼠水浸束缚应激性(water immersion and restraint stress,WRS)胃溃疡模型,按Guth标准计算溃疡指数(ulcer index,UI),应用免疫组织化学检测Keap1、Nrf2、HO-1在胃黏膜组织中的定位和表达情况。结果表明,WRS处理7 h后,大鼠胃黏膜出现明显的点线状出血;WRS处理前后,Keap1、Nrf2、HO-1蛋白均主要定位于大鼠胃底腺细胞胞质中,其中Nrf2在溃疡周围胃底腺细胞胞核中也有表达;WRS处理7 h后,Keap1表达量显著降低(P0.05),于7 d后恢复至正常水平,而HO-1表达量显著升高(P0.05),于3 d后恢复至正常水平;Nrf2恢复3 d后表达量显著升高(P0.05),随后出现下降。WRS处理后,Keap1、Nrf2、HO-1在大鼠胃黏膜中的定位和表达量均出现变化,提示Keap1/Nrf2/HO-1信号通路可能在应激性胃溃疡的发生及发展过程中发挥了一定抗应激作用。
[Abstract]:To investigate the role of Kelch like epichlorohydrin associated protein-1 Kelch-like epichlorohydrin-associated protein 1 Keap1 / nuclear factor E2 related factor 2(nuclear factor-E2-related factor 2nrf2nrf2 / heme oxygenase 1 HO-1 in the pathogenesis and development of stress gastric ulcer. A rat model of gastric ulcer induced by water immersion and restraint stress was established. The ulcer index was calculated according to Guth standard. The localization and expression of Keap1Nrf2HO-1 in gastric mucosa were detected by immunohistochemistry. The results showed that after 7 h of WRS treatment, there were obvious dot-line hemorrhage in gastric mucosa of rats. Before and after WRS treatment, Keap1Nrf2HO-HO-1 protein was mainly located in the cytoplasm of rat fundus glandular cells. The expression of Nrf2 in the nuclei of gastric fundus gland cells around ulcers was significantly decreased after 7 h treatment, and returned to normal level 7 days later. However, the expression of HO-1 increased significantly (P0.05A), and the expression level of Nrf2 increased significantly after 3 days, and then decreased. The localization and expression of Nrf2HO-1 in gastric mucosa of rats were all changed after WRS treatment. The results suggest that Keap1/Nrf2/HO-1 signaling pathway may play an antistress role in the occurrence and development of stress gastric ulcer.
【作者单位】：江苏大学医学院;江苏农林职业技术学院;
【基金】：国家自然科学基金(编号:81300287) 江苏省自然科学基金(编号:BK2011499;BK20140541) 江苏大学高级人才项目(编号:12JDG084)
【分类号】：R573.1

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