肥胖相关基因与叉头转录因子在非酒精性脂肪肝大鼠模型肝脏中的表达

发布时间：2018-05-20 02:14

本文选题：叉头转录因子类 + 非酒精性脂肪肝　；参考：《重庆医学》2017年30期

【摘要】：目的研究肥胖相关基因(FTO)与叉头转录因子O1(FoxO1)蛋白在非酒精性脂肪肝模型大鼠肝脏中的表达水平。方法通过饲喂高能量和高脂肪的大鼠饲料制备非酒精性脂肪肝动物模型,然后采集大鼠的血液和肝脏组织,测定其肝脏指数和血液生化指标,包括三酰甘油(TG)、总胆固醇(TC)、丙氨酸氨基转移酶(AST)、天冬氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL);对大鼠进行肝脏病理检测;采用免疫组织化学法测定FTO蛋白和FoxO1蛋白在大鼠肝脏中的表达水平。结果 8周后,模型组大鼠的肝脏质量、体质量和肝脏指数3项指标均高于对照组大鼠,差异有统计学意义(P0.05);模型组大鼠的AST、ALT、LDL、ALP、TG和TC均高于对照组大鼠,差异有统计学意义(P0.05);模型组大鼠的HDL低于对照组大鼠,差异有统计学意义(P0.05);模型组大鼠肝小叶部分产生脂肪变性、炎症,对照组大鼠则没有;模型组大鼠的FTO蛋白和FoxO1蛋白在肝脏中的表达积分高于对照组大鼠,差异有统计学意义(P0.05)。结论 FTO与FoxO1一起相互作用,可能扰乱正常的能量和脂肪代谢。
[Abstract]:Objective to study the expression level of obesity related gene (FTO) and fork head transcription factor O1 FoxO1 protein in the liver of non-alcoholic fatty liver model rats. Methods the animal model of non-alcoholic fatty liver was made by feeding high-energy and high-fat rats. The blood and liver tissues were collected and the liver index and blood biochemical index were measured. It included triglyceride TGG, total cholesterol TCX, alanine aminotransferase (ASTT), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), high density lipoprotein (HDL) and low density lipoprotein (LDL). The expression of FTO protein and FoxO1 protein in rat liver was determined by immunohistochemical method. Results after 8 weeks, the liver mass, body weight and liver index in the model group were higher than those in the control group (P 0.05), and the TG and TC of the model group were higher than those of the control group. The HDL of the model group was lower than that of the control group (P 0.05), the model group had fatty degeneration and inflammation in the hepatic lobules, but the control group had not. The expression of FTO protein and FoxO1 protein in the liver of the model group was significantly higher than that of the control group (P 0.05). Conclusion the interaction between FTO and FoxO1 may disrupt the normal energy and fat metabolism.
【作者单位】：海南省人民医院消化内科;
【基金】：海南省卫生与计划生育委员会基金资助项目(14A210192) 海南省自然科学基金资助项目(814310) 海南省中药现代化专项(2015ZY02)
【分类号】：R-332;R575

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