酒精性肝损伤小鼠在损伤过程中细胞凋亡时间点研究

发布时间：2018-05-20 06:50

本文选题：酒精性肝损伤 + Hoechst染色　；参考：《中国临床药理学杂志》2017年21期

【摘要】：目的研究小鼠酒精性肝损伤过程中肝细胞凋亡的变化规律。方法按照体重将健康KM雄性小鼠随机分为2组:对照组(n=10),模型组(n=80)。模型组给予56°白酒(7.5μL·g~(-1)·d~(-1))灌胃,持续8周。分别于1,2,3,4,5,6,7,8周脱臼处死小鼠,取肝组织;对照组小鼠等量蒸馏水灌胃后处死。进行苏木素-伊红染色(HE)和Hoechst_33258染色,检测小鼠酒精性肝损伤中肝细胞的凋亡变化;荧光显微镜下辨认和计数正常肝细胞和凋亡肝细胞,计算细胞凋亡率。结果第3周,模型组小鼠发生急性肝损伤改变,表现为炎症细胞浸润加剧;到第8周,进展为慢性肝损伤,表现为肝组织水样变,脂肪空泡。第1,2,3周,模型组的凋亡率分别为(9.28±0.41)%,(13.20±0.81)%,(20.43±0.97)%,与对照组的(1.38±0.18)%比较,差异均有统计学意义(均P0.05)。第8周末,凋亡率达到(51.99±1.70)%,与对照组相比,差异有统计学意义(P0.01)。结论小鼠酒精性肝损伤程度与肝细胞凋亡的发生发展密切相关。
[Abstract]:Objective to study the changes of hepatocyte apoptosis during alcoholic liver injury in mice. Methods healthy km male mice were randomly divided into two groups according to their body weight: control group (n = 10) and model group (n = 80). The model group was treated with 56 掳liquor (7.5 渭 L / g) D ~ (- 1) ~ (-1) by gastric perfusion for 8 weeks. The mice were killed after 8 weeks dislocated and the control group were killed by the same amount of distilled water. Hematoxylin-eosin staining (HEH) and Hoechst_33258 staining were used to detect the changes of hepatocyte apoptosis in alcoholic liver injury in mice. The normal hepatocytes and apoptotic hepatocytes were identified and counted under fluorescence microscope and the apoptosis rate was calculated. Results in the model group, acute liver injury occurred in the third week, and the inflammatory cell infiltration was aggravated in the model group, and in the 8th week, it developed into chronic liver injury, with the appearance of liver tissue watery change and fat vacuole. The apoptotic rate of the model group was 13.20 卤0.81% and 20.43 卤0.97% respectively, which was significantly higher than that of the control group (1.38 卤0.18%). At the end of the 8th week, the apoptotic rate reached 51.99 卤1.70. The difference was statistically significant compared with the control group (P 0.01). Conclusion the degree of alcoholic liver injury is closely related to the occurrence and development of hepatocyte apoptosis in mice.
【作者单位】：河南科技大学医学院肝损伤与修复分子医学重点实验室;
【基金】：国家自然科学基金资助项目(81201558) 河南省高校科技创新团队基金资助项目(181RTSTHN026) 河南省科技攻关基金资助项目(172102310693) 河南科技大学创新团队基金资助项目(2015XTD003)
【分类号】：R575

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