NR2B通过mBDNF参与肠易激综合征内脏高敏感的发生

发布时间：2018-05-21 11:35

本文选题：NRB + 成熟脑源性神经营养因子　；参考：《山东大学学报(医学版)》2017年07期

【摘要】：目的在神经系统,NMDA受体(NMDARs)NR2B亚基参与内脏高敏感的发生,本研究探讨在肠黏膜,该NR2亚型是否通过m BDNF(m BDNF)参与肠易激综合征内脏高敏感的发生。方法共纳入32例参与者,检测肠黏膜NR2B及m BDNF的表达水平。用Pearson线性相关分析NR2B与m BDNF表达水平的关系;用Spearman秩相关分析m BDNF表达水平与腹部疼痛评分的关系;以NMDA受体激动剂、NMDA受体阻滞剂、NR2B特异性阻滞剂干预细胞,ELISA法检测培养基中m BDNF蛋白表达水平的变化。结果腹泻型肠易激综合征(IBS-D)组肠黏膜组织NR2B及m BDNF蛋白表达水平均明显高于对照组(NR2B:90.22±30.79 vs 63.47±28.19,P=0.016;m BDNF:89.91±20.95 vs 56.53±22.25,P0.001),且两者表达水平呈正相关。m BDNF表达水平与腹部疼痛评分呈正相关。NMDA受体活化后可诱导m BDNF表达增加,该作用可完全被NMDA受体阻滞剂、NR2B特异性阻滞剂抑制。结论 NR2B亚基通过诱导m BDNF的表达参与IBS-D患者内脏高敏感的发生。
[Abstract]:Objective in the nervous system, the NMDA receptor (NMDARs) NR2B subunit is involved in the occurrence of visceral hypersensitivity. This study is to investigate whether the NR2 subtype is involved in the occurrence of visceral hypersensitivity in irritable bowel syndrome by M BDNF (m BDNF). Methods a total of 32 participants were included to detect the expression level of intestinal mucous membrane NR2B and m BDNF. The relationship between R2B and m BDNF expression level; the relationship between M BDNF expression level and abdominal pain score by Spearman rank correlation; NMDA receptor agonist, NMDA receptor blocker, NR2B specific blocker interfering cells, ELISA method to detect the expression level of M BDNF protein in the culture medium. Results the intestinal mucosa of diarrhea type irritable bowel syndrome The expression level of NR2B and m BDNF protein was significantly higher than that in the control group (NR2B:90.22 + 30.79 vs 63.47 + 28.19, P=0.016, m BDNF:89.91 + 20.95 vs 56.53 + 22.25, P0.001), and the expression level of the two was positively correlated with the abdominal pain score. It is inhibited by NMDA receptor blocker and NR2B specific blocker. Conclusion NR2B subunit is involved in the occurrence of visceral hypersensitivity in IBS-D patients by inducing m BDNF expression.
【作者单位】：山东大学齐鲁医院消化内科;
【基金】：国家自然科学基金(81370495)
【分类号】：R574.4

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