高压氧预处理对大鼠肠缺血再灌注机械屏障损伤的影响

发布时间：2018-05-22 09:05

本文选题：高压氧 + 缺血再灌注　；参考：《山东医药》2015年01期

【摘要】：目的探讨高压氧预处理对肠缺血再灌注机械屏障损伤的影响及其机制。方法按随机数字表法将30只大鼠分为假手术组、缺血再灌注组和高压氧预处理组各10只。采用肠系膜上动脉无创性暂时阻断45 min、恢复肠系膜血流再灌注1 h的方法构建大鼠肠缺血再灌注损伤模型。最后进行血清TNF-α、肠组织髓过氧化物酶测定、肠道通透性和组织学的观察。结果假手术组、缺血再灌注组和高压氧预处理组空肠推进长度、空肠推进率、血清TNF-α水平、肠组织髓过氧化物酶活性差异均有统计学意义(P均0.01)。缺血再灌注组黏膜下水肿、淤血,大量炎性细胞浸润,肠绒毛破坏,上皮细胞坏死,紧密连接、中间连接部分融合、模糊不清或消失;高压氧预处理组黏膜下血管轻微充血,少量炎性细胞浸润,微绒毛基本正常,可见清晰的紧密连接、中间连接和桥粒。结论缺血再灌注后大鼠肠道机械屏障出现不同程度的破坏,高压氧预处理可以通过其抗炎作用从而减轻大鼠肠机械屏障的缺血再灌注损伤。
[Abstract]:Objective to investigate the effect and mechanism of hyperbaric oxygen preconditioning on mechanical barrier injury of intestinal ischemia reperfusion. Methods Thirty rats were randomly divided into three groups: sham operation group, ischemia reperfusion group and hyperbaric oxygen preconditioning group. The rat model of intestinal ischemia-reperfusion injury was established by temporary non-invasive occlusion of superior mesenteric artery for 45 min and restoration of mesenteric blood flow for 1 h. Finally, serum TNF- 伪, intestinal myeloperoxidase, intestinal permeability and histology were observed. Results in sham operation group, ischemia reperfusion group and hyperbaric oxygen preconditioning group, there were significant differences in jejunal propulsion length, jejunum propulsion rate, serum TNF- 伪 level and myeloperoxidase activity in intestinal tissue (P < 0.01). In the ischemia-reperfusion group, submucosal edema, congestion, massive inflammatory cell infiltration, intestinal villi destruction, epithelial cell necrosis, tight junction, partial fusion of the middle junctions, ambiguity or disappearance, and mild hyperemia in the hyperbaric oxygen preconditioning group, A small number of inflammatory cells infiltrated, microvilli basically normal, clear tight junction, intermediate junction and desmosome. Conclusion the intestinal mechanical barrier was damaged in different degree after ischemia-reperfusion in rats. Hyperbaric oxygen preconditioning can reduce the ischemia-reperfusion injury of intestinal mechanical barrier through its anti-inflammatory effect.
【作者单位】：武警四川总队医院成都分院;沧州医学高等专科学校;武警医学院附属医院;
【分类号】：R574

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