TNBS诱导炎症性肠病大鼠结肠神经元tau蛋白磷酸化以及COX-2表达的变化

发布时间：2018-05-22 11:18

本文选题：炎症性肠病 + Tau蛋白　；参考：《中国病理生理杂志》2015年06期

【摘要】：目的:探讨三硝基苯磺酸(trinitrobenzene sulfonic acid,TNBS)诱导炎症性肠病(inflammatory bowel disease,IBD)模型大鼠结肠神经元tau蛋白磷酸化和环氧合酶2(COX-2)表达的变化。方法:30只健康雄性成年Wistar大鼠随机分为对照组、IBD模型组和TNBS组,每组10只,IBD模型组以TNBS乙醇连续灌肠14 d造模,对照组和TNBS组分别以等量生理盐水和TNBS灌肠;观察大鼠的一般情况和结肠病理组织学改变,用anti-Hu作为神经元标志以免疫荧光法检测结肠黏膜下神经元的数量变化,免疫荧光双染色检测结肠黏膜下神经元COX-2和磷酸化tau231、tau262的表达变化。结果:与对照组比较,IBD模型组大鼠结肠黏膜下神经元数量明显减少(P0.05),神经元tau蛋白磷酸化程度明显升高(P0.05),而TNBS组大鼠神经元数量与对照组相比无显著差别;对照组和TNBS组大鼠结肠黏膜下神经元几乎不表达COX-2,IBD模型组大鼠结肠神经细胞胞核和胞浆中均有COX-2的表达,与对照组和TNBS组相比有显著差异(P0.05)。结论:TNBS乙醇诱导IBD模型大鼠结肠黏膜下神经元减少,可能与tau蛋白高度磷酸化及COX-2表达有关。
[Abstract]:Aim: to investigate the changes of tau protein phosphorylation and cyclooxygenase 2COX-2 expression in colon neurons of inflammatory bowel disease model rats induced by trinitrobenzene sulfonic bowel. Methods 30 healthy male adult Wistar rats were randomly divided into two groups: control group (n = 10) and TNBS group (n = 10). Each group (n = 10) was treated with TNBS ethanol for 14 days. The control group and TNBS group were treated with the same amount of normal saline and TNBS respectively. The general situation and pathological changes of colon in rats were observed. The number of submucous neurons in colon was detected by immunofluorescence using anti-Hu as a neuronal marker. The expression of COX-2 and phosphorylated tau231 tau262 in submucous neurons of colon were detected by double immunofluorescence staining. Results: compared with the control group, the number of submucosal neurons in the IBD group decreased significantly, and the phosphorylation of tau protein in the neurons increased significantly, but there was no significant difference in the number of neurons between the TNBS group and the control group. The expression of COX-2 in the nucleus and cytoplasm of colon neurons in the control group and the TNBS group was almost undetectable, compared with the control group and the TNBS group. There was a significant difference in the expression of COX-2 between the control group and the TNBS group (P 0.05). Conclusion the decrease of submucosal neurons in IBD rats induced by 1: TNBS ethanol may be related to the high phosphorylation of tau protein and the expression of COX-2.
【作者单位】：潍坊医学院应用药理学山东省高校重点实验室;潍坊医学院药理学教研室;
【基金】：山东省自然科学基金资助项目(No.ZR2009DM024;No.ZR2011HM043) 山东省高等学校科技计划(No.J11LF33) 2013年山东省政府公派留学项目山东省医药卫生计划(No.2014WS0478)
【分类号】：R574.62

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