β-半乳糖苷酶在急性胰腺炎模型大鼠血清中的活性变化水平及意义

发布时间：2018-05-23 19:15

本文选题：急性胰腺炎 + 大鼠　；参考：《广东医学》2016年18期

【摘要】：目的探讨β-半乳糖苷酶在急性胰腺炎模型大鼠血清中的活性变化水平及意义。方法雄性Wistar大鼠30只,体重200~250 g,随机分为3组。分别为空白对照组(CON组,n=10)、轻症急性胰腺炎组(MAP组,n=10)和重症急性胰腺炎模型组(SAP组,n=10)。分别予以开腹后仅翻动胰十二指肠关腹、主胰管内注射1%牛磺胆酸钠溶液(0.1 m L/100 g体重)、主胰管内注射5%牛磺胆酸钠溶液(0.1 m L/100 g体重)。全自动生化仪检测各组血清淀粉酶、脂肪酶、丙氨酸氨基转移酶及钙离子水平。取胰腺组织4%多聚甲醛固定后切片,HE染色后光镜下观察3组胰腺组织病理学改变并评分,免疫组织化学法检测各组肿瘤坏死因子-α(TNF-α)表达情况。酶联免疫吸附(ELISA)法检测各组血清中β-半乳糖苷酶活性、血清白细胞介素-6水平及胰腺组织β-半乳糖苷酶活性水平。结果与CON组相比较,MAP组和SAP组大鼠血清淀粉酶、脂肪酶、丙氨酸氨基转移酶、白细胞介素-6水平及胰腺组织β-半乳糖苷酶活性明显升高(P0.05),血清钙离子及血清β-半乳糖苷酶活性水平明显降低(P0.05)。光镜下可见腺叶组织坏死严重、毛细血管扩张充血、组织间隙增宽、大量炎性细胞浸润等,胰腺组织病理评分明显升高(P0.05)、TNF-α表达明显增加。与MAP组相比较,SAP组大鼠血清淀粉酶、脂肪酶、丙氨酸氨基转移酶、白细胞介素-6水平及胰腺组织β-半乳糖苷酶活性明显升高(P0.05),血清钙离子及血清β-半乳糖苷酶活性水平明显降低(P0.05),胰腺组织坏死严重、病理评分升高、TNF-α表达增加。结论血清β-半乳糖苷酶活性水平在大鼠急性胰腺炎时明显下降,且下降程度与病情严重程度有关。
[Abstract]:Objective to investigate the level and significance of 尾-galactosidase in serum of acute pancreatitis rats. Methods Thirty male Wistar rats, weighing 200,250g, were randomly divided into 3 groups. The results were as follows: control group (Con group), mild acute pancreatitis group (map group) and severe acute pancreatitis model group (SAP group). After laparotomy, only pancreaticoduodenal closure was turned over. 1% sodium taurocholate solution was injected into the main pancreatic duct with 0.1 mL / 100 g body weight, and 5% sodium taurocholate solution was injected into the main pancreatic duct with 0.1 mL / 100 g body weight. The levels of serum amylase, lipase, alanine aminotransferase and calcium ion were detected by automatic biochemical instrument. The histopathological changes of pancreas were observed under light microscope after 4% paraformaldehyde fixation and the expression of TNF- 伪 was detected by immunohistochemical method. Serum 尾 -galactosidase activity, serum interleukin-6 level and pancreatic tissue 尾 -galactosidase activity were detected by Elisa. Results compared with CON group, serum amylase, lipase and alanine aminotransferase in map group and SAP group were compared. The level of interleukin-6 and the activity of 尾 -galactosidase in pancreatic tissue increased significantly (P 0.05), and the levels of serum calcium and 尾 -galactosidase decreased significantly (P 0.05). Under the light microscope, there were serious necrosis of glandular lobes, hyperemia of capillaries, widening of tissue space and infiltration of inflammatory cells. The expression of TNF- 伪 in pancreatic tissue was increased significantly. Compared with MAP group, serum amylase, lipase, alanine aminotransferase in SAP group, The level of interleukin-6 and the activity of 尾 -galactosidase in pancreatic tissue were significantly increased (P 0.05), the levels of serum calcium and 尾 -galactosidase were significantly decreased, the pancreatic tissue necrosis was serious, and the expression of TNF- 伪 was increased. Conclusion the level of serum 尾-galactosidase decreased significantly in acute pancreatitis in rats, and the degree of decline was related to the severity of the disease.
【作者单位】：海南省农垦总医院急诊科;
【分类号】：R576;R-332

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