Inflammatory bowel disease NADPH oxidase Oxidative stress No

  本文关键词：NADPH氧化酶Nox1和Duox2在小鼠肠炎中的表达和意义，由笔耕文化传播整理发布。

NADPH氧化酶Nox1和Duox2在小鼠肠炎中的表达和意义

Significance of NADPH oxidases Nox1 and Duox2 expression in mouse colitis

[1] [2] [3] [4] [5] [6]

Mo-Li Yang, Hong-Qian Li, Xiao-Zhen Li, Ya-Lin Jiang, Meng-Lu Chen, Qiang Gao（ Department of Gastroenterology, the First Affiliated Hospital of He＇nan University of Science and

河南科技大学第一附属医院消化内科,河南省洛阳市471023

文章摘要：目的：探讨NADPH氧化酶Noxl和Duox2在小鼠肠炎发病中的表达及意义.方法：将6-8周龄的C57BL/6,小鼠随机分为正常组、1.5%葡聚糖硫酸钠（dextran sulphate sodium,DSS）组和3.0%DSS组,每组10只,正常组正常饮水,1.5%和3.0%DSS组分别自由饮用含有1.5%或者3.0%DSS的饮水,共6d建立小鼠急性肠炎模型.通过疾病活动指数（disease activity index,DAI）评分、结肠长度测定和HE染色等方法评估肠道炎症程度.利用生化方法检测血清丙二醛（malondialdehyde,MDA）含量以及结肠组织中氧化型谷胱甘肽/还原型谷胱甘肽（oxidized glutathione/glutathione,GSSG/GSH）比率等氧化应激指标,以评估机体氧化应激程度.采用免疫组织化学方法和实时定量PCR技术分别检测小鼠结肠组织中NADPH氧化酶Nox1和Duox2蛋白及mRNA表达情况.结果：正常组小鼠无肠炎,1.5%DSS组、3.0%DSS组小鼠分别呈轻度和重度肠炎.氧化应激指标（MDA、GSSG/GSH）在1.5%DSS组升高,而3.0%DSS组进一步升高（均P〈0.05）.NADPH氧化酶Nox1与Duox2蛋白和mRNA在不同炎症程度时表达不同：Nox1蛋白和mRNA在正常组呈高表达,在1.5%DSS组表达下调（P〈0.05）,在3.0%DSS组进一步下调（P〈0.05）;Duox2蛋白和mRNA在1.5%DSS组表达较正常组明显上调（P〈0.05）,而在3.0%DSS组表达下调至正常水平.结论：Nox1主要在维持肠道正常生理功能中发挥作用,而Duox2除了维持正常生理功能外,可能还积极地参与肠炎发病过程.

Abstr：AIM：To investigate the significance of expression of NADPH oxidases Nox1 and Duox2 in the pathogenesis of mouse colitis.METHODS：Six-to-eight-week-old C57BL/6mice were randomly divided into a control group,a 1.5%dextran sulfate sodium（DSS）group and a 3%DSS group（n- 10 for each group）.Colitis was induced in mice by giving1.5%DSS or 3%DSS in drinking water for 6d,and mice in the control group was given drinking water only.Disease activity index（DAI）,colon length and inflammatory score of the colon were observed.Oxidative stress indicators,malondialdehyde（MDA） in serum and oxidized glutathione/glutathione（GSSG/GSH） ratio in colon tissue,were measured by biochemical methods.The protein and mRNA expression of Noxl and Duox2 in colon tissue of mice was evaluated by immunohistochemistry and quantitative real-time PCR,respectively.RESULTS：There was no colitis in the control group,while mild and severe enteritis was found in mice in the 1.5%DSS group and3.0%DSS group,respectively.Oxidative stress（MDA and GSSG/GSH） was enhanced along with the increased concentration of DSS（P 0.05 for both）.The expression of Noxl and Duox2 protein and mRNA was different with the severity of inflammation：Noxl protein and mRNA was highly expressed in normal colon epithelial cells,but down-regulated in the 1.5%DSS group（P〈 0.05）,and further reduced in the 3%DSS group（P 〈0.05）;Duox2 protein and mRNA expression was increased（P〈 0.05）in mice in the 1.5%DSS group compared to the control group,but returned to the control level in the 3%DSS group.CONCLUSION：Noxl is mainly involved in the functional and structural maintenance of normal colonic epithelium,while Duox2 may actively participate in the inflammatory pathogenesis besides the physiological process of the colon.

文章关键词：

Keyword：:Inflammatory bowel disease NADPH oxidase Oxidative stress Nox1 Duox2

课题项目：国家自然科学基金资助项目,No.81370487

  本文关键词：NADPH氧化酶Nox1和Duox2在小鼠肠炎中的表达和意义，，由笔耕文化传播整理发布。