趋化因子受体CX3CR1在大鼠重症急性胰腺炎中的表达和意义

发布时间：2018-05-27 08:36

本文选题：趋化因子 + CXCR　；参考：《西安交通大学学报(医学版)》2015年02期

【摘要】：目的探讨趋化因子受体CX3CR1在重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠模型中的表达,判断其能否作为SAP发生的预测指标。方法 40只Sprague-Dawley大鼠随机分为假手术组(sham operation,SO)(n=20)和SAP模型组(n=20),分别于制模后6、12、24、48h处死大鼠,应用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)法检测SAP大鼠血清中CX3CR1的变化,同时应用免疫组织化学法检测SAP大鼠胰腺、肺脏及肾脏组织中CX3CR1蛋白的表达。结果大鼠SAP制模成功后,血清中CX3CR1的表达逐渐升高,24h达最高(542.4pg/mL),明显高于SO组(P0.05)。大鼠SAP组的胰腺、肺脏和肾脏组织中均有CX3CR1的表达,且明显高于SO组,其中胰腺和肺脏组织中CX3CR1表达在制模24h达高峰,肾脏组织中CX3CR1表达在制模48h达高峰。结论趋化因子受体CX3CR1可能为预测SAP严重程度的有效指标之一,值得进一步深入研究。
[Abstract]:Objective to investigate the expression of chemokine receptor (CX3CR1) in severe acute patients with severe acute pancreatitis (SAP) and determine whether it can be used as a predictor of SAP. Methods Forty Sprague-Dawley rats were randomly divided into sham operation group (n = 40) and SAP model group (n = 20). The rats were killed at 612t 24 h after modeling. The changes of CX3CR1 in serum of SAP rats were detected by enzyme-linked immunosorbent assaysis-ELISA-linked enzyme linked immunosorbent assay (Elisa). Immunohistochemical method was used to detect the expression of CX3CR1 protein in pancreas, lung and kidney of SAP rats. Results after SAP modeling, the expression of CX3CR1 in serum gradually increased to a peak of 542.4 PG / mL at 24 h, which was significantly higher than that in so group (P 0.05). The expression of CX3CR1 in pancreas, lung and kidney in SAP group was significantly higher than that in so group. The expression of CX3CR1 in pancreas and lung reached its peak at 24 h, and CX3CR1 expression in kidney reached the peak at 48 h. Conclusion the chemokine receptor CX3CR1 may be one of the effective indexes to predict the severity of SAP, and it is worthy of further study.
【作者单位】：宁夏医科大学临床学院;宁夏医科大学总医院消化内科;宁夏吴忠市新区医院B超室;宁夏医科大学总医院综合科;
【基金】：国家自然科学基金(No.81260086)~~
【分类号】：R576

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