枯否细胞通过组织因子对大鼠急性胰腺炎凝血系统变化的影响

发布时间：2018-05-27 09:02

本文选题：枯否细胞 + 组织因子　；参考：《重庆医科大学学报》2015年03期

【摘要】：目的:观察大鼠急性胰腺炎(acute pancreatitis,AP)组织因子(tissue factor,TF)及凝血系统的变化,探讨枯否细胞(kupffer cells,KCs)通过组织因子对大鼠AP凝血系统的作用。方法:108只SD大鼠完全随机分为假手术(sham operation,SO)组、轻症胰腺炎(mild acute pancreatitis,MAP)组、重症胰腺炎(severe acute pancreatitis,SAP)组、SO+氯化钆(Gd Cl3)组、MAP+Gd Cl3组、SAP+Gd Cl3组。Gd Cl3预处理组在诱发AP模型前24 h予尾静脉注射Gd Cl3(10 mg/kg,0.1 ml),分别用1.5%和5%的牛黄胆酸钠胰胆管逆行注射诱导MAP和SAP模型。建模后或假手术组分别于术后6、12、24 h处死大鼠,检测其血清中淀粉酶及凝血项的变化;ELISA法检测血清中TF的含量;分离并培养KCs,采用Western blot法检测其TF的蛋白含量;免疫组化检测肝脏中TF蛋白的表达;HE染色检测胰腺组织病理损伤情况。结果:MAP和SAP组大鼠血清中淀粉酶含量明显高于SO组(P0.01),与MAP+Gd Cl3组、SAP+Gd Cl3组各相应时间点比较无明显差异。同时间点MAP组和SAP组较SO组血清中TF含量明显升高,MAP+Gd Cl3组和SAP+Gd Cl3组较MAP组和SAP组明显降低(P0.05)。MAP和SAP组大鼠肝脏中TF蛋白表达较SO组表达明显增加。MAP+Gd Cl3和SAP+Gd Cl3组KCs中TF蛋白含量较MAP组和SAP组明显降低(P0.05)。SAP组较MAP和SO组大鼠血清凝血酶原时间(prothrombin time,PT)、活化部分凝血活酶时间(activated partial thromboplastin time,APTT)、纤维蛋白原浓度(fibrinogen,FIB)明显升高,SAP+Gd Cl3组较SAP明显降低(P0.05)。大鼠胰腺病理切片显示,MAP组胰腺间质水肿,SAP组胰腺呈凝固性坏死,伴有大量炎症细胞浸润,MAP+Gd Cl3和SAP+Gd Cl3组分别较MAP和SAP组病理损伤明显减轻。结论:AP时KCs中TF表达明显增加,并能导致凝血象的改变,抑制KCs功能可有效降低AP时TF的表达,减少凝血象的改变,从一定程度上可降低AP的发生和发展。
[Abstract]:Objective: To observe the changes of tissue factor (tissue factor, TF) and coagulation system of acute pancreatitis (AP) in rats and to explore the effect of Kupffer cell (Kupffer cells, KCs) on the coagulation system of rat AP through the tissue factor. Methods: 108 SD rats were completely divided into sham operation group and mild pancreatitis. Group Ancreatitis, MAP), group severe acute pancreatitis (SAP), SO+ gadolinium chloride (Gd Cl3) group, MAP+Gd Cl3 group, SAP+Gd group preconditioning group (10, 0.1, 0.1). The sham operation group died of 6,12,24 h after the operation to detect the changes of amylase and coagulation in serum, ELISA assay was used to detect the content of TF in serum; KCs was isolated and cultured, Western blot method was used to detect the protein content of TF; immunohistochemistry was used to detect the expression of TF protein in the liver; HE staining was used to detect the pathological damage of the pancreas. Results: MA The content of amylase in serum of P and SAP rats was significantly higher than that in group SO (P0.01). There was no significant difference in the corresponding time points between the MAP+Gd Cl3 group and the SAP+Gd Cl3 group. The content of TF in the MAP group and SAP group was obviously higher than that of the SO group. The expression of white expression in group SO was significantly higher than that in.MAP+Gd Cl3 and SAP+Gd Cl3 group, and the content of TF protein in group KCs was significantly lower than that of MAP and SAP groups (P0.05).SAP group than MAP and serum prothrombin time, activated partial thromboplastin time and fibrinogen concentration. FIB) significantly increased and SAP+Gd Cl3 group was significantly lower than SAP (P0.05). Pathological section of pancreas showed that pancreatic interstitial edema in group MAP, pancreatic necrosis in group SAP and infiltration of inflammatory cells in group SAP, MAP+Gd Cl3 and SAP+Gd Cl3 group were significantly less than MAP and other groups. Changes in coagulation and inhibition of KCs function can effectively reduce the expression of TF and reduce the changes of coagulation in AP, which can reduce the occurrence and development of AP to a certain extent.
【作者单位】：重庆医科大学附属第二医院肝胆外科;重庆市第五人民医院肝胆外科;
【基金】：国家自然科学基金青年基金资助项目(编号:81301618)
【分类号】：R576

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