5-羟色胺对肠炎小鼠结肠组织中GRP78表达的影响

发布时间：2018-05-28 12:00

本文选题：-羟色胺 + 炎症性肠病　；参考：《郑州大学学报(医学版)》2016年02期

【摘要】：目的:观察5-羟色胺(5-HT)对肠炎小鼠结肠组织葡萄糖调节蛋白78(GRP78)表达的影响。方法:将7~8周龄的C57BL/6小鼠随机分为6组(n=8):正常对照组,低、高剂量5-HT组(分别给予1.0、2.0 mg/kg的5-HT灌肠,每3 d 1次),低、高剂量DSS组(分别饮用10或25 g/L DSS溶液),DSS+5-HT组(给予10 g/L DSS+1.0mg/kg 5-HT),于第6天结束时处死小鼠。通过疾病活动指数评分、结肠长度测定和HE染色等方法评估肠道炎症程度,采用实时定量PCR技术检测小鼠结肠组织中IL-6、TNF-α和GRP78 mRNA的表达水平,采用免疫组化法检测小鼠结肠组织中GRP78蛋白的表达。结果:正常对照组,低、高剂量5-HT组小鼠无肠炎,低剂量DSS组有轻微肠炎,高剂量DSS组和DSS+5-HT组有严重肠炎。IL-6和TNF-αmRNA的表达水平随炎症程度而增加。GRP78mRNA和蛋白在正常对照组、低剂量5-HT组和低剂量DSS组中的表达水平差异无统计学意义(P0.05),在高剂量5-HT组、DSS+5-HT组和高剂量DSS组,尤其是高剂量DSS组表达增高(P0.05)。结论:5-HT可能通过上调GRP78蛋白的表达激活内质网应激反应,参与肠道炎症反应。
[Abstract]:Aim: to observe the effect of 5-HT on the expression of glucose-regulated protein 78GRP78 in colonic tissue of colitis mice. Methods: C57BL/6 mice aged 7 to 8 weeks were randomly divided into 6 groups: normal control group, low dose 5-HT group, high dose 5-HT group (1. 0 mg/kg 5-HT enema, once every 3 days, low dose). High dose DSS group (drinking 10 or 25 g / L DSS solution respectively) DSS 5-HT group (10 g / L DSS 1.0mg/kg 5-HTG) were given 10 g / L DSS 1.0mg/kg 5-HT, and the mice were killed at the end of the 6th day. The degree of intestinal inflammation was evaluated by disease activity index score, colonic length measurement and HE staining. The expression of IL-6 TNF- 伪 and GRP78 mRNA in colonic tissue of mice was detected by real-time quantitative PCR technique. The expression of GRP78 protein in mouse colon was detected by immunohistochemical method. Results: normal control group, low and high dose 5-HT group had no enteritis, low dose DSS group had mild enteritis, high dose DSS group and DSS 5-HT group had severe enteritis. IL-6 and TNF- 伪 mRNA expression level increased with inflammation degree in normal control group. There was no significant difference in the expression level between the low dose 5-HT group and the low dose DSS group. The expression of P0. 05 was increased in the high dose 5-HT group and the high dose DSS group, especially in the high dose DSS group. Conclusion the reaction of endoplasmic reticulum stress may be activated by up-regulating the expression of GRP78 protein, which may be involved in intestinal inflammation.
【作者单位】：河南科技大学第一附属医院(河南科技大学临床医学院)消化内科;河南科技大学第一附属医院(河南科技大学临床医学院)检验科;
【基金】：国家自然科学基金面上项目81370487
【分类号】：R574

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