可溶性上皮钙黏蛋白检测在HBV感染相关疾病中的应用研究

发布时间：2018-05-30 21:29

本文选题：可溶性上皮钙黏蛋白 + 慢性乙型肝炎　；参考：《重庆医科大学》2017年硕士论文

【摘要】：研究目的:大量研究证实可溶性上皮钙黏蛋白(souble E-cadherin,s E-cadherin)是促进各类疾病的发生、发展进程的一个关键的调控因子。慢性乙型肝炎病毒(hepatitis B virus,HBV)感染是导致全世界肝脏相关疾病发病率和致死率的首要因素,目前已知全球约有2.4亿人慢性HBV感染者,并且其中25%死于该类疾病的后期并发症。本项研究诣在更好地探究血清s E-cadherin在参与HBV感染相关疾病进程中的应用价值。研究方法:采用酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)试剂盒分别检测了125例正常对照组(Healthy individuals)和113例慢性乙型肝炎(chronic hepatitis B,CHB)患者、101例乙肝后硬化(Cirrhosis)患者、75例肝细胞癌(hepatocellular cancer,HCC)患者血清中的s E-cadherin和干扰素-γ(interferon-γ,IFN-γ)水平,并用SPSS19.0软件分析s E-cadherin、INF-γ水平分别与HBV感染相关疾病的相关性。研究结果:血清中s E-cadherin值在HBV相关疾病患者组中显著高于健康对照组(均P0.0001)。在慢性乙型肝炎和乙肝后肝硬化患者的血清中s E-cadherin表达水平与INF-γ、丙氨酸氨基转移酶(alanine transarninase,ALT)、门冬氨酸氨基转移酶(aspartate aminotransferase,AST)水平呈显著正相关。而在肝细胞癌患者血清中s E-cadherin浓度仅于与ALT、AST水平呈显著正相关。结论:s E-cadherin可能是评估HBV相关疾病进程的一个重要血清标志物。
[Abstract]:Objective: a large number of studies have confirmed that soluble E-cadherin (E-cadherin) is a key regulatory factor to promote the occurrence and development of various diseases. Chronic hepatitis B virus (HBV) infection is the leading cause of morbidity and mortality of liver-related diseases in the world. There are about 240 million chronic HBV infections in the world, and 25% of them die from the late complications of these diseases. The purpose of this study is to explore the application value of serum s E-cadherin in the process of HBV infection-related diseases. Methods: enzyme linked immunosorbent Elisa kit was used to detect 75 patients with hepatocellular carcinoma (HCC), including 125 healthy individuals (normal control group) and 113 patients with chronic hepatitis B hepatitis (CHB) and 101 patients with chronic hepatitis B virus (CHB), and 75 patients with hepatocellular carcinoma (HCC) were examined by enzyme-linked immunosorbent assay (Elisa) kit. The levels of s E-cadherin and interferon- 纬 (IFN- 纬), SPSS19.0 software was used to analyze the correlation between the level of s E-cadherin-纬 and HBV infection-related diseases. Results: the serum s E-cadherin level in patients with HBV related diseases was significantly higher than that in healthy controls (P 0. 0001). The expression of s E-cadherin in serum of patients with chronic hepatitis B and posthepatitic cirrhosis was positively correlated with the levels of INF- 纬, alanine transarninase, aspartate aminotransferase and aspartate aminotransferase. However, the serum s E-cadherin level was only positively correlated with alt AST level in HCC patients. Conclusion: E-cadherin may be an important serum marker for evaluating the progression of HBV related diseases.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R512.62

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