酒精性肝病患者肠道菌群变化与肝功能的相关性研究

发布时间：2018-06-06 03:15

本文选题：酒精性肝病 + 肠道菌群　；参考：《广东医学》2017年16期

【摘要】：目的探讨酒精性肝病(ALD)患者肠道菌群变化与肝功能的相关性。方法采用全自动生化分析仪检测31例ALD患者(ALD组)血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活力和总胆红素(TBil)含量。采用16S r DNA实时荧光定量PCR技术对粪便肠道菌群中大肠杆菌、双歧杆菌、肠球菌和乳杆菌进行定量分析。同时选择20例健康体检者作为对照组。结果与对照组相比,ALD组ALT、AST活力及TBil含量显著升高(P0.05),分别达到(43.47±23.57)U/L、(40.07±26.55)U/L和(17.47±10.71)μmol/L。ALD组粪便中双歧杆菌为(5.36±1.62)拷贝数/g湿粪,较对照组[(6.87±1.36)拷贝数/g湿粪]明显减少,两组比较差异有统计学意义(P0.05);而大肠杆菌、肠球菌和乳杆菌与对照组比较,差异无统计学意义(P0.05)。两组人群的双歧杆菌与ALT(r=-0.64)、AST(r=-0.45)呈负相关(P0.01),与TBil呈负相关(r=-0.11,P0.05)。结论 ALD患者存在肠道菌群失调,对肠道菌群中双歧杆菌进行定量分析可为早期发现ALD患者提供可靠依据,同时为ALD的治疗提供新的思路。
[Abstract]:Objective to investigate the relationship between intestinal flora and liver function in patients with alcoholic liver disease (ALD). Methods Serum alanine aminotransferase (alt), aspartate aminotransferase (AST) activity and total bilirubin (T) content were measured by automatic biochemical analyzer in 31 patients with ALD. The quantitative analysis of Escherichia coli, Bifidobacterium, Enterococcus and Lactobacillus in fecal microflora was carried out by 16s r DNA real-time quantitative PCR. At the same time, 20 healthy persons were selected as control group. Results compared with the control group, the activity of AST and the content of TBil in the ALD group were significantly higher than those in the control group (P 0.05), which reached 43.47 卤23.57 U / L (40.07 卤26.55)U/L) and 17.47 卤10.71 渭 mol/L.ALD (P < 0.05), respectively. The number of copies of Bifidobacterium was 5.36 卤1.62copy / g wet feces, which was significantly lower than that of the control group [6.87 卤1.36] copies / g wet feces. The difference between the two groups was statistically significant (P 0.05), but that of Escherichia coli, Enterococcus and Lactobacillus was not significantly different from that of the control group. There was a negative correlation between Bifidobacterium and TBil in the two groups, and there was a negative correlation between Bifidobacterium and TBil. Conclusion there is intestinal flora disorder in patients with ALD. Quantitative analysis of bifidobacterium in intestinal flora can provide reliable basis for early detection of ALD patients and provide a new idea for the treatment of ALD.
【作者单位】：青岛大学附属医院重症医学科;青岛大学医学院细胞与分子生物学实验室;青岛大学医学院护理学院;青岛大学医学院医学营养研究所;青岛大学附属医院手术室;
【基金】：国家自然科学基金资助项目(编号:81573137) 达能营养中心膳食营养研究与宣教基金(编号:DIC2014-03)
【分类号】：R575

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