溃疡性结肠炎发病特征及肠黏膜和血清中Beclin1蛋白的表达及其意义

发布时间：2018-06-08 02:33

本文选题：溃疡性结肠炎 + 年代变化　；参考：《青岛大学》2016年博士论文

【摘要】：第一部分溃疡性结肠炎临床特征的回顾研究目的:近年来,溃疡性结肠炎(Ulcerative colitis,UC)在我国发病率日益增高,各地区发病情况各不相同,本文旨在探讨近十年来潍坊市中医院UC的住院率、发病特征、复发等情况的年代变化。方法:调取2005年2月至2015年1月期间于潍坊市中医院确诊并治疗的溃疡性结肠炎的患者。统计这些患者的性别、年龄、发病部位、发病类型、发病程度以及内镜分级、是否复发等方面数据,分析这些近10年临床特征的变化情况及住院率情况。结果:自2005年至2015年,我院溃疡性结肠炎住院率呈上升趋势,从0.99‰增至1.57‰,共有286例患者诊断为UC,男女比例为:1.47:1,中位年龄40.22±16.12岁(前5年平均年龄:37.51±15.54岁,近5年平均年龄41.41±11.26岁)。通过分析发现近5年比前5年在发病类型及发病部位上有显著改变,近5年发病类型慢性持续型增多,住院患者中全结肠发病的比例增多(p0.05);UC的复发与发病部位、病情、内镜分级、发病类型、居住地显著相关(p0.05),与年龄、性别无相关性。结论:我院近10年UC住院率有所增高。UC患者以男性发病较多,年龄以30岁左右居多,发病年龄有增长趋势。UC复发与发病部位、病情、内镜分级、发病类型、居住地密切相关。UC患者的发病类型、发病部位有所改变。第二部分溃疡性结肠炎患者肠黏膜组织及血清中Beclin1蛋白表达的意义和相关性研究目的:本实验通过对UC患者病变肠组织以及血清中Beclin1的表达情况,探讨UC发病中是否存在自噬异常,并进一步探讨与其临床病理特征之间的关系。方法:收集2013年9月至2014年11月我院诊治UC患者75例,对照组为同期检查的IBS患者37例。体外保存两组肠道的活检黏膜组织,并采集血清,组织中及血清中Beclin1表达的检测采用ELISA法;组织中Beclin1的表达情况再采用IHCA法同步验证;应用RT-PCR检测两组组织中Beclin1m RNA的表达量以及按病情分组,观察三组组织中Beclin1m RNA的表达情况。结果:ELISA法检测UC患者的肠组织和血清中Beclin1均表达增高,并且与IHCA检测结果一致。UC患者Beclin1的表达较对照组明显增高(P0.05),且Beclin1的表达量与病情程度、内镜下分级、病理分级成正相关,差异有统计学意义(P0.05)。UC患者肠黏膜组织中Beclin1 m RNA的表达量明显高于对照组,并随病情严重程度的增高而表达增高(P0.05)。结论:从血清学、组织学以及分子生物学的角度检测Beclin1结果一致,均发现溃疡性结肠炎患者Beclin1表达增高,溃疡性结肠炎存在自噬异常,且不同的发病特征(病情程度、病变范围、内镜及病理分级)的各组之间的自噬水平有显著差异,与病情严重程度呈正相关。
[Abstract]:In the first part, the clinical characteristics of ulcerative colitis are reviewed. The incidence of ulcerative colitis (Ulcerative colitis, UC) in our country is increasing in recent years, and the incidence of disease in each region is different. This paper aims to discuss the rate of hospitalization in the Weifang Hospital of Traditional Chinese Medicine UC in the last ten years, the characteristics of the disease, and the change of the recurrence. Patients with ulcerative colitis, diagnosed and treated in Weifang Hospital of Traditional Chinese Medicine from February 2005 to January 2015, were used to analyze the sex, age, location, type of disease, degree of onset, endoscopic classification and recurrence of these patients, and analyze the changes in clinical characteristics and hospitalization rate of these 10 years. Results: 20 From 05 to 2015, the hospitalization rate of ulcerative colitis in our hospital was on the rise, from 0.99 per thousand to 1.57 per thousand. A total of 286 patients were diagnosed as UC. The proportion of men and women was 1.47:1, the median age was 40.22 + 16.12 years old (the average age of the first 5 years: 37.51 + 15.54 years, and the average age 41.41 + 11.26 years of the 5 years). There was a significant change in the location of the disease, the incidence of chronic persistent type increased in the last 5 years and the proportion of all colonic diseases in the hospitalized patients increased (P0.05); the recurrence of the disease and the location of the disease, the disease, the classification of endoscopy, the type of disease, the place of residence (P0.05), and the age and sex were not related. Conclusion: the rate of hospitalization in UC in our hospital for the last 10 years was higher than that of the.UC patients. Most of the onset of sexual disease is about 30 years old, the age of onset has a trend of growth in.UC recurrence and location, disease, endoscopic classification, type of disease, closely related to the type of.UC patients, the location of the disease is changed. The significance and correlation of the expression of Beclin1 protein in the intestinal mucus tissue and serum in second parts of ulcerative colitis patients Objective: To investigate the expression of Beclin1 in the intestinal tissue and serum of UC patients, to explore the existence of autophagic abnormalities in the pathogenesis of UC, and to further explore the relationship with its clinicopathological features. Methods: 75 cases of UC were treated in our hospital from September 2013 to November 2014, and the control group was 37 of the IBS patients in the same period. Two groups of intestinal biopsies were preserved in vitro, and serum was collected, the expression of Beclin1 in tissue and serum was detected by ELISA. The expression of Beclin1 in tissue was confirmed by IHCA, and RT-PCR was used to detect the expression of Beclin1m RNA in the tissues of the two groups and to group the Beclin1m RNA in the three groups. Results: the expression of Beclin1 in the intestinal tissue and serum of UC patients was increased by ELISA method, and the expression of Beclin1 in.UC patients was significantly higher than that of the control group (P0.05), and the expression of Beclin1 was positively correlated with the degree of disease, the classification of endoscopy, and the pathological grade, and the difference was statistically significant (P0.05).UC patients enteric. The expression of Beclin1 m RNA in mucous tissue was significantly higher than that in the control group and increased with the severity of the disease (P0.05). Conclusion: the results of Beclin1 were consistent in serology, histology and molecular biology, and all found the higher expression of Beclin1 in ulcerative colitis and the abnormal autophagy in ulcerative colitis. There was a significant difference in the autophagy level among the groups with the same disease characteristics (disease severity, lesion size, endoscopy and pathological grading), and positively correlated with the severity of the disease.
【学位授予单位】：青岛大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：R574.62

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