门脉注射聚氨酯-四氢呋喃溶液制备犬门脉高压症模型

发布时间：2018-06-08 04:28

本文选题：门脉高压症 + 动物模型　；参考：《生物医学工程学杂志》2015年03期

【摘要】：介绍一种利用聚氨酯高分子材料和有机溶剂四氢呋喃配制的溶液制备犬门脉高压症动物模型的方法,并评价成模效果。选用西安本地杂种犬12只,取上腹部小切口,经门静脉推注8%(W/V)的聚氨酯-四氢呋喃溶液建立门脉高压症模型。分别在造模前、造模中、造模后4、8、12周测量实验犬门静脉压力,以造模前门静脉压力作为正常参考值,评估模型建立效果。结果显示经门静脉注射聚氨酯-四氢呋喃溶液后门静脉压力可立即升至造模前的2.5倍,4周后门静脉压力维持在造模前的1.5倍以上。该法制备犬门脉高压症模型操作简单、死亡率低、门静脉压力维持稳定,尤其是腹腔粘连极轻,门静脉的结构和位置保持正常,非常适合外科手术治疗门脉高压症方面的研究。
[Abstract]:An animal model of portal hypertension in dogs was established by using polyurethane polymer and organic solvent tetrahydrofuran solution, and the effect of modeling was evaluated. A portal hypertension model was established in 12 Xi'an local mongrel dogs with a small upper abdominal incision and injection of 8 W / V solution of polyurethane tetrahydrofuran via portal vein. The portal vein pressure of experimental dogs was measured before, during and after modeling for 12 weeks. The portal vein pressure before modeling was taken as the normal reference value to evaluate the effect of model establishment. The results showed that portal vein pressure increased to 2.5 times of that before modeling immediately after injection of polyurethane tetrahydrofuran solution via portal vein, and the portal vein pressure was maintained above 1.5 times of that before modeling after 4 weeks. This method has the advantages of simple operation, low mortality, stable portal vein pressure, light adhesion of abdominal cavity, normal portal vein structure and location, and is very suitable for surgical treatment of portal hypertension.
【作者单位】：西安交通大学第一附属医院肝胆外科;西安交通大学先进外科技术与工程研究所;陕西省再生医学与外科工程技术研究中心;陕西省人民医院肿瘤外科;
【基金】：卫生部部属(管)医院临床学科重点资助项目(2010105) 中央高校基本科研业务费专项基金资助项目(XKJC2013003) 国家自然科学基金科学仪器专项资助项目(81127005)
【分类号】：R575.2;R-332

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