HLA-Ⅱ类基因多态性与幽门螺杆菌感染相关性的Meta分析

发布时间：2018-06-09 17:22

本文选题：HLA-DQA1 + HLA-DQB1　；参考：《华北理工大学》2017年硕士论文

【摘要】：目的通过对涉及HLA-Ⅱ类基因多态性与H.pylori感染相关性的文献进行Meta分析,证明二者之间有无联系,揭示遗传因素中HLA-Ⅱ类基因对H.pylori感染是否存在影响。方法检索中英文数据库,包括中国知网、万方、维普、中国生物医学文献数据库、PUBMED、MEDLINE、OVID、COCHRANE等医学数据库。检索时间范围1995年1月—2016年6月。检索的文献涉及HLA-Ⅱ类基因多态性与H.pylori感染的关系。根据制定的纳入排除标准,对检索到的文献进行筛检。仔细阅读筛检后纳入的文献,进行数据提取和质量评价。数据提取时将需要进行Meta分析的数据分为H.pylori感染组(病例组)和H.pylori未感染组(对照组);质量评价则按照STREGA报告规范进行量化评分,3分文献质量过低。衡量结果的效应指标有I2、Z值、P值、OR和95%CI。根据各位点的森林图,制作Meta分析结果汇总表。行敏感性分析、亚组分析。绘制漏斗图对发表偏倚进行定性评估,同时使用Egger’s法进行定量评估。结果1 DQA1共纳入了16个基因位点,其中DQA1*0102位点,H.pylori感染组基因频率低于H.pylori未感染组(P=0.0008,OR95%CI:0.72(0.59,0.87)),即DQA1*0102为抵抗H.pylori感染的抵抗基因。同时按地区的亚组分析显示,DQA1*0102在亚洲地区(P=0.03,OR95%CI:0.68(0.49,0.96))是H.pylori感染的抵抗基因,而欧洲地区不再是抵抗基因(P=0.43)。DQA1*0103位点H.pylori感染组基因频率高于H.pylori未感染组(P=0.009,OR95%CI:1.35(1.08,1.69)),即DQA1*0103是H.pylori感染的易感基因。DQA1*0104、0301位点是H.pylori感染的易感基因(P=0.0002,OR95%CI:1.95(1.37,2.76))、(P=0.04,OR95%CI:1.32(1.01,1.71))。但敏感性分析(改变统计学模型)后,DQA1*0104(P=0.06)、0301(P=0.11)位点不再是H.pylori感染的易感基因。2DQB1共纳入了19个基因位点,其中DQB1*03032是避免H.pylori感染的抵抗基因(P=0.0003,OR95%CI:0.55(040,0.76)),并且纳入的文献地区都为亚洲,所以是亚洲人群的抵抗基因。DQB1*04、0401是H.pylori感染的易感基因(P=0.02,OR95%CI::2.56(1.14,5.75))、(P=0.0004,OR95%CI:1.87(1.32,2.65))。DQB1*0602、0604位点是H.pylori感染的抵抗基因(P=0.02,OR95%CI::0.69(0.50,0.94))、(P=0.0009,OR95%CI:0.50(0.30,0.84)),但敏感性分析(改变统计学模型)后,DQB1*0602、0604位点不再具有统计学差异(P=0.08)、(P=0.24)。3 DRB1共纳入了38个基因位点,其中DRB1*08,11,二组比较(P=0.03,OR95%CI:1.61(1.05,2.49))、(P=0.04,OR95%CI:1.43(1.02,2.00)),说明DRB1*08是H.pylori感染的易感基因,但敏感性分析(改变统计学模型)后,DRB1*08、11不再是H.pylori感染的易感基因(P=0.07)、(P=0.10)。另外对DRB1*11位点进行亚组分析(按H.pylori诊断的方法分组)。使用联合试验诊断H.pylori的DRB1*11位点是否与H.pylori感染有关系,尚需更多的数据(P=0.05,OR95%CI:2.33(1.01,5.39))。DRB1*12位点,二组比较没有统计学差异(P=0.28)。但经亚组分析(按地区)后,显示亚洲地区DRB1*12为H.pylori感染的抵抗基因(P=0.04,OR95%CI:0.45(0.21,0.96))。欧洲地区DRB1*12是否与为H.pylori感染有关尚需更多数据(P=0.05,OR95%CI:2.62(0.99,6.90))。DRB1*1502位点亚组分析(按地区)后,欧洲地区DRB1*1502是否与H.pylori感染有关系,尚需更多的数据(P=0.05,OR95%CI:3.15(0.98,10.15))。结论1 DQA1*0102与H.pylori感染存在相关性,是亚洲人群避免H.pylori感染的抵抗基因。2 DQA1*0103与H.pylori感染存在相关性,是H.pylori感染的易感基因。3 DQB1*04与H.pylori感染存在相关性,是H.pylori感染的易感基因。4DQB1*0401与H.pylori感染存在相关性,是H.pylori感染的易感基因。5在亚洲人群中DQB1*03032与H.pylori感染存在相关性,是避免H.pylori感染的抵抗基因。6尚未发现DRB1位点与H.pylori感染存在明确的相关性。
[Abstract]:Objective through Meta analysis of the literature related to the correlation between HLA- II gene polymorphism and H.pylori infection, it is proved that there is no connection between the two and the influence of the HLA- II gene on the H.pylori infection in the genetic factors. Methods the Chinese database, including the Chinese knowledge network, the Wanfang, VIP, and the Chinese biomedical literature database, is retrieved. Medical databases such as PUBMED, MEDLINE, OVID, COCHRANE, and other medical databases. Retrieval time range from January 1995 to June 2016. The documents retrieved involved the relationship between the HLA- type II gene polymorphism and H.pylori infection. According to the inclusion criteria formulated, the retrieved literature was screened. The documents included in the screening were carefully read, and the data were extracted and the quality was obtained. Meta analysis was divided into H.pylori infection group (case group) and H.pylori uninfected group (control group) when the data was extracted. The quality evaluation was quantified according to the STREGA report standard, and the quality of the 3 points was too low. The effect indexes of the results were I2, Z value, P value, OR and 95%CI. based on the forest map of each point, to make Meta. Analysis of the results of the analysis, line sensitivity analysis, subgroup analysis. Draw the funnel map to make a qualitative assessment of the publication bias, and use the Egger 's method for quantitative evaluation. Results 1 DQA1 included 16 gene loci, of which the DQA1*0102 locus, the H.pylori infection group was lower than the H.pylori uninfected group (P=0.0008, OR95%CI:0.72 (0.59,0.87)). DQA1*0102 is a resistance gene that resists H.pylori infection. According to regional subgroup analysis, DQA1*0102 is the resistance gene of H.pylori infection in Asia (P=0.03, OR95%CI:0.68 (0.49,0.96)), while the European region is no longer the resistance gene (P=0.43).DQA1*0103 point H.pylori infection group, and the gene frequency is higher than that of the H.pylori infection group (P=0.0). 09, OR95%CI:1.35 (1.08,1.69)), that is, DQA1*0103 is the susceptible gene of H.pylori infection, the.DQA1*01040301 locus is the susceptible gene of H.pylori infection (P=0.0002, OR95%CI:1.95 (1.37,2.76)), (P=0.04, OR95%CI:1.32 (1.01,1.71)). But after sensitivity analysis, the 0301 locus is no longer an infection. The susceptibility gene.2DQB1 is included in 19 loci, in which DQB1*03032 is the resistance gene to avoid H.pylori infection (P=0.0003, OR95%CI:0.55 (040,0.76)) and is included in the literature region for Asia, so the resistance gene.DQB1*040401 of the Asian population is the susceptible gene of H.pylori infection (P=0.02, OR95%CI:: 2.56 (1.14,5.75)), (P=0.). 0004, OR95%CI:1.87 (1.32,2.65)).DQB1*06020604 site is the resistance gene of H.pylori infection (P=0.02, OR95%CI:: 0.69 (0.50,0.94)), (P=0.0009, OR95%CI:0.50 (0.30,0.84)), but after sensitivity analysis (change statistical model), DQB1*06020604 loci no longer have statistical difference (P=0.08), including 38 gene loci. DRB1*08,11, two groups (P=0.03, OR95%CI:1.61 (1.05,2.49)), (P=0.04, OR95%CI:1.43 (1.02,2.00)), indicating that DRB1*08 is a susceptible gene for H.pylori infection, but after sensitivity analysis (change statistical model), DRB1*08,11 is no longer the susceptible gene of H.pylori infection (P=0.07). Ylori diagnostic methods. The use of a combined test to diagnose the DRB1*11 loci of H.pylori is associated with H.pylori infection, and more data (P=0.05, OR95%CI:2.33 (1.01,5.39)).DRB1*12 loci are needed, and there is no statistical difference between the two groups (P=0.28). However, the Asian region DRB1*12 is shown to be H.pylori infection after subdivision (according to the region). Resistance genes (P=0.04, OR95%CI:0.45 (0.21,0.96)). Whether DRB1*12 in Europe is related to H.pylori infection still needs more data (P=0.05, OR95%CI:2.62 (0.99,6.90)) subgroup analysis of.DRB1*1502 (according to region), whether DRB1*1502 in Europe is related to H.pylori infection and need more data (P=0.05,). Conclusion there is a correlation between 1 DQA1*0102 and H.pylori infection. It is the correlation between the resistance gene.2 DQA1*0103 of the Asian population to avoid H.pylori infection and the infection of H.pylori. The susceptibility gene of H.pylori infection is related to.3 DQB1*04 and H.pylori infection, and the susceptible basis of H.pylori infection is associated with.4DQB1*0401 and infection. The susceptibility gene.5 of H.pylori infection is associated with DQB1*03032 and H.pylori infection in Asian population. It is a resistance gene to avoid H.pylori infection,.6 has not yet found a clear correlation between the DRB1 locus and H.pylori infection.
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R573

【参考文献】