肝再生增强因子通过增加调节T细胞数量保护小鼠急性肝损伤

发布时间：2018-06-16 08:23

本文选题：肝再生增强因子(ALR) + 急性肝损伤　；参考：《细胞与分子免疫学杂志》2015年01期

【摘要】：目的研究肝再生增强因子(ALR)保护急性肝损伤的作用及机制。方法将30只BALB/c小鼠随机分为正常对照组、急性肝损伤组和ALR干预组。急性肝损伤组小鼠按2 m L/kg体质量的剂量予以腹腔注射500 m L/L四氯化碳(CCl4)矿物油溶液1次。ALR干预组于CCl4注射前8 h尾静脉注射ALR质粒,正常对照组注射等量生理盐水注射液。收集小鼠肝组织和血液标本,HE染色观察肝组织病理形态学变化;生化法检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)水平;流式细胞术检测肝组织中调节性T细胞(Treg)的数量,实时定量PCR(qRT-PCR)检测肝组织Foxp3、ALR、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)的mRNA水平。结果 ALR干预组小鼠肝组织中ALR mRNA表达水平显著高于急性肝损伤组和正常对照组,急性肝损伤组与正常对照组相比无明显差异;流式细胞术检测结果显示ALR干预组小鼠肝组织中CD25+Foxp3+Treg/CD4+T细胞为(5.90±0.10)%,高于急性肝损伤组的(4.23±0.46)%和正常对照组的(2.93±0.74)%,急性肝损伤组显著高于正常对照组;ALR干预组小鼠肝组织中Foxp3 mRNA表达水平高于急性肝损伤组和正常对照组,急性肝损伤组高于正常对照组,但差异无统计学意义;ALR干预组与急性肝损伤组相比,IL-6 mRNA、TNF-αmRNA表达水平降低,而与正常对照组相比无明显变化,急性肝损伤组与正常对照组相比,IL-6 mRNA、TNF-αmRNA表达水平明显升高。结论 ALR通过上调Treg数量保护小鼠急性肝损伤,可能与Treg下调肝脏IL-6、TNF-α表达有关。
[Abstract]:Objective to study the effect and mechanism of liver regeneration enhancement factor (ALR) in the protection of acute liver injury. Methods 30 BALB/c mice were randomly divided into normal control group, acute liver injury group and ALR intervention group. The mice of acute liver injury group were injected with 500 m L/L four chlorinated carbon (CCl4) mineral oil solution in 1.ALR intervention group by the dose of 2 m L/kg body mass. ALR plasmids were injected 8 h caudal vein before injection of CCl4, and normal control group was injected with equal amount of saline injection. The liver tissue and blood samples were collected in mice. The pathological changes of liver tissue were observed by HE staining; serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) level was detected by biochemical method; flow cytometry was used to detect the liver tissue. The number of segmental T cells (Treg), real-time quantitative PCR (qRT-PCR) detection of Foxp3, ALR, interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF- a) mRNA levels. Results the expression level of ALR mRNA in the liver tissues of the mice in the ALR intervention group was significantly higher than that in the acute liver injury group and the normal control group, and the acute liver injury group was not clearly compared with the normal control group. The results of flow cytometry showed that the CD25+Foxp3+Treg/CD4+T cells in the liver tissue of the ALR intervention group were (5.90 + 0.10)%, higher than (4.23 + 0.46)% of the acute liver injury group and (2.93 + 0.74)% of the normal control group. The acute liver injury group was significantly higher than that of the normal control group, and the expression level of Foxp3 mRNA in the liver tissues of the ALR intervention group was higher than that of the acute liver injury group. The liver injury group and the normal control group, the acute liver injury group was higher than the normal control group, but the difference was not statistically significant. Compared with the acute liver injury group, the expression level of IL-6 mRNA and TNF- a mRNA decreased, but there was no significant change compared with the normal control group, and the acute liver injury group was compared with the normal control group, and the IL-6 mRNA, TNF- alpha mRNA expression level was compared with the normal control group. Conclusion ALR can protect mice from acute liver injury by increasing the quantity of Treg, which may be related to the downregulation of IL-6 and TNF- alpha by Treg.
【作者单位】：首都医科大学附属北京佑安医院临检中心;首都医科大学附属北京佑安医院北京市肝病研究所;首都医科大学附属北京佑安医院人工肝中心;
【基金】：国家自然科学基金(81300349) 北京市科技新星计划(Z131107000413016) 北京市自然科学基金(7144216) 北京市留学人员科技活动择优资助项目(2014) 佑安肝病艾滋病基金(BJYAH-2011-017)
【分类号】：R575.3

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