急性肝损伤模型鼠血清中microRNAs绝对定量与肝损伤程度的相关性研究

发布时间：2018-06-17 14:02

本文选题：肝损伤 + microRNAs　；参考：《四川大学学报(医学版)》2017年03期

【摘要】：目的观察四氯化碳(CCl_4)诱导的大鼠急性肝损伤模型和对乙酰氨基酚(APAP)诱导的小鼠急性肝损伤模型血清中microRNAs(miR-122、miR-451、miR-92a、miR-192)的绝对定量随时间的变化情况,并分析血清中microRNAs绝对定量与对乙酰氨基酚诱导的小鼠急性肝损伤组织病理评分之间的相关性。方法建立CCl_4(1.5mL/kg)诱导的大鼠急性肝损伤模型和APAP(160mg/kg)诱导的小鼠急性肝损伤模型,收集建模过程中各时间点的血清,通过MiRbay~(TM) SV miRNA检测试剂盒对血清中microRNAs的含量进行绝对定量,探究血清中microRNAs的变化情况。同时收集APAP诱导的小鼠急性肝损伤模型中各时间点的肝损伤组织,进行组织病理染色、组织病理评分;随后对小鼠血清中microRNAs的绝对定量与肝损伤组织病理评分进行相关性分析。结果在两种急性肝损伤模型中,均发现血清中miR-122和miR-192的绝对定量在肝损伤8h或2h就出现明显的升高,在24h左右维持在最高的水平,72h逐渐下降至正常水平。miR-92a在CCl_4诱导的大鼠急性肝损伤模型中,出现波动性的变化,无明显的变化规律,miR-451则出现了轻微下降的趋势。miR-92a和miR-451在APAP诱导的小鼠肝损伤进展中,均有逐渐下降的趋势,在48h到达最低点,随后开始恢复。相关性分析发现,小鼠血清中miR-122和miR-192的绝对定量与APAP诱导的小鼠肝损伤组织病理评分(DILI-PSS)呈现良好的正相关(分别为r=0.741 3,P0.05;r=0.788 3,P0.01)。结论血清中microR-122和microR-192的绝对定量在大鼠或小鼠急性肝损伤后24h左右达峰,72h降低,并且与APAP诱导的小鼠急性肝损伤组织病理评分具有良好的相关性。
[Abstract]:Objective to observe the changes of serum microRNAsmiR-121 miR-451miR-92a miR-1922) in the acute liver injury model of rats induced by CClSIV) and the acute liver injury model induced by paracetamol APAP1), and to observe the changes of the absolute quantity of microRNAsmiR-121 miR-451 miR-92a miR-1922 in serum of rats with acute liver injury induced by acetaminophen (APAP). The relationship between the absolute quantity of microRNAs in serum and the pathological score of acute liver injury in mice induced by paracetamol was analyzed. Methods the acute liver injury model of rats induced by CCl4L / kg and acute liver injury model of mice induced by APAPN 160 mg / kg were established. Serum samples were collected at different time points during the modeling process. The levels of microRNAs in serum were measured by SV miRNA assay kit. To explore the changes of microRNAs in serum. At the same time, the tissue of acute liver injury in mice induced by APAP was collected at different time points, and then the relationship between the absolute quantity of microRNAs in serum and the pathological score of liver injury was analyzed. Results in both acute liver injury models, the absolute quantification of miR-122 and miR-192 in serum was significantly increased at 8h or 2h after liver injury. At the highest level at about 24 h, it gradually decreased to the normal level. MiR-92a showed the change of volatility in CClS4 induced acute liver injury model in rats. Without obvious change, miR-451 showed a slight downward trend. MiR-92a and miR-451 showed a decreasing trend in the progression of liver injury induced by APAP, reached the lowest point at 48h, and then recovered. The correlation analysis showed that the absolute quantification of miR-122 and miR-192 in serum was positively correlated with the pathological score of liver injury in mice induced by APAP (r = 0.741 3, P 0.05 0. 788 3 P 0.01). Conclusion the absolute quantification of microR-122 and microR-192 in serum decreased at about 24 hours after acute liver injury in rats or mice, and had a good correlation with the pathological score of acute liver injury induced by APAP in mice.
【作者单位】：四川大学生命科学学院;四川大学实验动物中心;
【分类号】：R-332;R575

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