非酒精性脂肪肝病大鼠血清短链脂肪酸水平的变化

发布时间：2018-06-19 05:18

本文选题：高脂饮食 + 非酒精性脂肪肝病　；参考：《安徽医科大学学报》2017年02期

【摘要】：目的探讨非酒精性脂肪肝病(NAFLD)大鼠血清短链脂肪酸(SCFA)水平的变化及意义。方法 36只雄性SD大鼠经过普通饲料适应性喂养1周后,随机分为两组,普通饮食组(NC组)和高脂饮食组(HF组),每组各18只。HF组给予高脂饲料喂养,NC组给予普通饲料喂养。分别于实验第4、8及12周每组各处死6只大鼠。生物化学法检测肝组织匀浆总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C);ELISA法检测肝组织匀浆白介素(IL)-6、肿瘤坏死因子(TNF)-α、IL-1β、IL-18;气相色谱法检测血清SCFA,主要包括乙酸、丙酸、正丁酸。结果与同期NC组相比,HF组肝组织匀浆中TG、TC及LDL-C含量升高,差异有统计学意义(P0.05);HDLC含量降低,差异有统计学意义(P0.05)。HF组第4、8及12周肝组织匀浆中IL-6、TNF-α、IL-1β、IL-18水平均较同期NC组升高,差异有统计学意义(P0.05)。与同期NC组相比,HF组血清中乙酸、丙酸及丁酸含量之和显著增加,差异有统计学意义(P0.05);其中,与同期NC组比较,HF组乙酸含量显著增加,差异有统计学意义(P0.05),丙酸和丁酸含量差异无统计学意义。结论 NAFLD大鼠血清SCFA水平升高,SCFA在NAFLD发病机制中可能产生一定作用。
[Abstract]:Objective to investigate the changes and significance of serum short chain fatty acid (SCFA) levels in rats with non alcoholic fatty liver disease (NAF LDD). Methods Thirty-six male Sprague-Dawley rats were randomly divided into two groups: normal diet group (NC group) and high fat diet group (HF group). 18 rats in each group were fed with high fat diet group (NC group). At the 8th and 12th week, 6 rats were killed in each group. Detection of liver homogenate total cholesterol (TCN), triglyceride (TGG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-CU) by biochemical method in liver tissue homogenate by Elisa Clear SCFA, mainly including acetic acid, Propionic acid. Results compared with NC group, the content of TGN TC and LDL-C in liver homogenate of HF group was higher than that of NC group, and the content of HDLC in liver tissue homogenate of HHF group was significantly lower than that of NC group, and the level of IL-6 TNF- 伪 IL-1 尾 IL-18 in liver tissue homogenate of HHF group was significantly higher than that in NC group at the 4th week and 12th week, and the level of IL-6 TNF- 伪 TNF- 伪 IL-1 尾 IL-18 in liver tissue homogenate of HHF group was higher than that in NC group. The difference was statistically significant (P 0.05). Compared with NC group, the sum of acetic acid, propionic acid and butyric acid in serum of HF group was significantly higher than that of NC group, and the difference was statistically significant (P 0.05), and the acetic acid content in HF group was significantly higher than that in NC group at the same time. The difference was statistically significant (P 0.05), but there was no significant difference in propionic acid and butyric acid content. Conclusion the elevated level of serum SCFA may play a role in the pathogenesis of NAFLD in NAFLD rats.
【作者单位】：安徽医科大学第一附属医院临床营养科;
【基金】：安徽省科技攻关计划项目(编号:1604a0802081)
【分类号】：R575

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