白介素-18、白介素-4在慢性丙型肝炎患者中的表达及意义研究

发布时间：2018-06-19 18:50

本文选题：慢性丙型肝炎 + 发病机制　；参考：《广西医科大学》2014年硕士论文

【摘要】：目的:观察慢性丙型肝炎（CHC）患者血清中的白介素-18（IL-18）、白介素-4（IL-4）的水平及其与丙型肝炎病毒载量（HCV RNA）、丙氨酸氨基转移酶（ALT）的关系，探讨IL-18、IL-4在CHC患者HCV病毒复制及致病机制中可能的作用。方法:收集2012年12月至2013年4月在广西医科大学第一附属医院就诊的CHC患者59例，健康对照组20例，所有CHC患者均符合2004年中华医学会肝病学分会联合传染病与寄生虫病学分会发布的《丙型肝炎防治指南》中CHC诊断标准。采用ELISA法、PCR-荧光探针法、全自动生化分析仪分别检测研究对象外周血清IL-18、IL-4、HCV RNA、ALT等指标。结果: （1）CHC患者组与健康对照组相比较，IL-18表达水平显著升高，，两组中位数分别为118.52pg/mL、11.09pg/mL，差异具有统计学意义（P=0.000）；IL-4表达水平在病例组与健康组间差异无统计学意义（P＞0.05）；IL-18/IL-4在CHC患者组中（M=28.27pg/mL）高于健康对照组（M=2.02pg/mL），差异具有统计学意义（P＜0.05）。（2）CHC患者组IL-18与IL-4两变量进行相关性分析，结果示两变量呈正相关（r=0.615）。（3）IL-18、IL-4表达在HCV RNA阳性组与阴性组间差异无统计学意义（P＞0.05）。（4）HCV RNA阳性患者病毒载量与IL-18、IL-4浓度分别进行相关性分析，结果均无显著相关性，r值分别为0.015、0.175。（5）病例组血清ALT水平与IL-18、IL-4水平分别进行相关性分析，结果无明显相关性，r值分别为0.191、0.235。结论: （1）慢性丙型肝炎疾病过程中IL-18明显升高，IL-18与疾病的发展存在一定的关系；（2）慢性丙型肝炎患者IL-18/IL-4失衡，Th1细胞优势可能在其发病中发挥一定的作用。
[Abstract]:Objective: to observe the serum levels of interleukin-18 (IL-18) and interleukin-4 (IL-4) in patients with chronic hepatitis C (CHCC) and their relationship with HCV RNA and alanine aminotransferase (alt). To explore the possible role of IL-18 IL-4 in HCV virus replication and pathogenesis in CHC patients. Methods: from December 2012 to April 2013, 59 CHC patients in the first affiliated Hospital of Guangxi Medical University and 20 healthy controls were collected. All patients with CHC were in accordance with the diagnostic criteria of CHC published in the guidelines for the Prevention and treatment of Hepatitis C, published by the Association of Hepatology and Hepatology of the Chinese Medical Association in 2004. Elisa and PCR- fluorescence probe were used to detect IL-18, IL-4, IL-4, HCV RNA, alt and so on. Results: the expression of IL-18 in CHC group was significantly higher than that in healthy control group. The median of the two groups was 118.52pg / mL 11.09pg / mL, the difference was statistically significant (P < 0.01). There was no significant difference in the expression of IL-4 between the two groups (P > 0.05). IL-18 / IL-4 in CHC group was 28.27pg / mL) higher than that in healthy control group (2.02 PG / mLL), the difference was significant (P < 0.05). The correlation between IL-18 and IL-4 in CHC group was analyzed. The results showed that there was no significant difference in the expression of IL-18 IL-4 between HCV RNA positive group and negative group (P > 0.05). Results there was no significant correlation between serum alt level and IL-18 IL-4 level. The results showed that there was no significant correlation between serum alt level and IL-18 IL-4 level. The results showed that there was no significant correlation between serum alt level and IL-18 IL-4 level. The results showed that there was no significant correlation between serum alt level and IL-18 IL-4 level. Conclusion: (1) the increase of IL-18 in patients with chronic hepatitis C has a certain relationship with the development of the disease, and the imbalance of IL-18 / IL-4 in patients with chronic hepatitis C may play a role in the pathogenesis of chronic hepatitis C.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R512.63

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