肝过表达hNPC1L1促进脂质过氧化

发布时间：2018-06-27 05:47

本文选题：hNPCL + 过表达　；参考：《浙江农业学报》2017年10期

【摘要】：利用体细胞核移植技术将构建的肝过表达h NPC1L1的小型猪胎儿成纤维细胞作为核供体细胞,构建了肝过表达h NPC1L1转基因巴马小型猪,PCR和RT-PCR鉴定表明h NPC1L1特异性地表达于肝组织。对转基因巴马小型猪进行了初步研究,脂质过氧化标记物丙二醛(MDA)检测表明:转基因猪MDA含量(7.51±0.09)nmol·m L-1显著高于非转基因猪(3.56±0.16)nmol·m L-1(P0.001),说明肝组织发生了严重的脂质过氧化。超氧化物歧化酶(SOD)活性检测表明:SOD活性为(131±4.61)U·mg-1显著低于非转基因猪[(229.2±4.39)U·mg-1](P0.001),说明肝脏清除自由基和抗氧化能力大大下降。试验揭示出在肝脏过表达h NPC1L1导致严重的脂质过氧化,表明NPC1L1在脂代谢紊乱和肝脏疾病中扮演着重要的角色,有望成为肝脏疾病治疗新的靶标。
[Abstract]:Using somatic cell nuclear transfer technique, the constructed porcine fetal fibroblasts which over-expressed hNPC1L1 were used as nuclear donor cells. PCR and RT-PCR analysis showed that hNPC1L1 was specifically expressed in the liver tissue of HNPC1L1 transgenic Bama mini-pig. The malondialdehyde (MDA), a lipid peroxidation marker, was detected in transgenic Bama miniature pigs. The results showed that the content of MDA in transgenic pigs (7.51 卤0.09) nmol m L-1) was significantly higher than that in non-transgenic pigs (3.56 卤0.16) nmol m L-1 (P0.001), indicating that serious lipid peroxidation occurred in liver tissue. The activity of superoxide dismutase (SOD) was (131 卤4.61) U mg-1 significantly lower than that of non-transgenic pigs [(229.2 卤4.39) U mg-1] (P0.001). The results showed that overexpression of hNPC1L1 in the liver resulted in severe lipid peroxidation, suggesting that NPC1L1 plays an important role in lipid metabolism disorders and liver diseases, and may become a new target for the treatment of liver diseases.
【作者单位】：吉林化工学院生物与食品工程学院;韶关学院英东生命科学院粤北生猪生产及疫病防控协同创新发展中心;吉林农业大学动物科学技术学院;吉林大学动物医学学院;
【基金】：国家科技支撑计划(2011BA115B02)
【分类号】：R575

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