骨髓间充质干细胞移植肝硬化大鼠肝脏超微结构、体视学参数和肝功能的变化

发布时间：2018-06-28 01:06

本文选题：肝硬化 + 骨髓　；参考：《中国组织工程研究》2015年50期

【摘要】：背景:间充质干细胞移植治疗肝硬化的疗效在学术界尚有一定的争议,对受体组织结构和功能的影响有待进一步研究。目的:探讨骨髓间充质干细胞对肝硬化大鼠肝脏超微结构、体视学参数和肝功能的影响。方法:构建大鼠肝硬化模型,并采用骨髓间充质干细胞移植治疗,透射电镜观察大鼠肝组织的超微结构,体视学分析软件分析模型组、治疗组、正常组大鼠肝窦的体视学参数变化,生化仪检测肝功能指标的改变。结果与结论:1模型大鼠肝细胞缺氧严重,许多线粒体破坏,核固缩明显,肝窦呈毛细血管化;骨髓间充质干细胞治疗组肝组织超微结构明显改善,肝细胞核基本正常,线粒体肿胀显著缓解,核膜孔堵塞现象有所减轻。2模型组大鼠肝窦数目明显减少,而肝窦总面积及平均直径明显增大,与治疗组及正常组比较差异有显著性意义(P0.05)。3与模型组比较,治疗组肝功能明显改善,白蛋白水平显著升高,谷丙转氨酶及直接胆红素水平显著下降(P0.05),但谷草转氨酶变化不明显(P0.05)。4结果提示骨髓间充质干细胞移植可改善肝硬化大鼠肝功能和肝组织结构,减轻肝窦病变,对肝硬化有一定的治疗作用。
[Abstract]:Background: the efficacy of mesenchymal stem cell transplantation in the treatment of liver cirrhosis is still controversial in academic circles, and the effect of mesenchymal stem cell transplantation on the structure and function of the recipient remains to be further studied. Aim: to investigate the effects of bone marrow mesenchymal stem cells (BMSCs) on liver ultrastructure, stereological parameters and liver function in cirrhotic rats. Methods: the liver cirrhosis model of rats was established, and bone marrow mesenchymal stem cell transplantation was used. The ultrastructure of rat liver tissue was observed by transmission electron microscope, and the model group and treatment group were analyzed by stereological analysis software. The changes of stereological parameters and liver function indexes were detected by biochemical instrument in normal rats. Results and ConclusionThe liver cells of the rat model of 1: 1 were seriously hypoxic, many mitochondria were destroyed, the nuclei were constricted obviously, the hepatic sinusoids were capillary, the ultrastructure of liver tissue was improved obviously and the liver nucleus was basically normal in the bone marrow mesenchymal stem cell treatment group. The swelling of mitochondria was alleviated significantly, the phenomenon of nuclear membrane pore blockage was alleviated, and the number of hepatic sinuses decreased, while the total area and mean diameter of hepatic sinuses increased significantly in the model group. Compared with the treatment group and the normal group, there was significant difference (P0.05). 3. Compared with the model group, the liver function of the treatment group was significantly improved, and the albumin level was significantly increased. The levels of alanine aminotransferase and direct bilirubin decreased significantly (P0.05), but the changes of glutamic oxalacetic transaminase were not obvious (P0.05). The results indicated that the transplantation of bone marrow mesenchymal stem cells could improve liver function and liver tissue structure and alleviate hepatic sinusoidal lesion in cirrhotic rats. It has certain therapeutic effect on liver cirrhosis.
【作者单位】：解放军第123医院南京军区肝病中心;
【基金】：南京军区医学科技创新经费资助项目重点课题(11Z012)~~
【分类号】：R575.2

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