S-腺苷蛋氨酸对奈乙硫氰酸酯致大鼠药物型胆汁淤积性肝病肝纤维化的保护作用

发布时间：2018-07-05 14:10

本文选题：肝硬化 + 胆汁淤积　；参考：《重庆医学》2015年26期

【摘要】：目的研究S-腺苷蛋氨酸(SAMe)对奈乙硫氰酸酯(ANIT)诱发大鼠药物型胆汁淤积性肝病肝纤维化的保护作用。方法将18只SD大鼠分为3组:A组(空白对照组)、B组(造模+NS)、C组(造模+SAMe),每组各6只,各组大鼠腹腔注射后72h处理。采用全自动生化分析仪检测各组肝脏血清学指标,并取肝脏组织行HE染色及MASSON染色观察肝脏病理组织学变化及胶原含量改变。结果 C组大鼠的血清学指标谷氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBiL)、直接胆红素(DBiL)、间接胆红素(IBiL)、谷胺酰转肽酶(γ-GGT)、碱性磷酸酶(ALP)、总胆汁酸(TBA)明显低于B组,3组之间比较差异均有统计学意义(P0.05);但3组之间总胆固醇(Chol)水平差异无统计学意义(P0.05)。C组大鼠的胶原纤维含量及胶原纤维面积比均明显低于B组,差异有统计学意义(P0.05)。结论 SAMe能通过改善药物型胆汁淤积性肝病的肝功能,降低胶原纤维含量及面积,进一步保护肝脏组织。
[Abstract]:Objective to study the protective effect of S-adenosylmethionine (SAMe) on hepatic fibrosis induced by neethylthiocyanate (ANIT) in rats with drug-induced cholestatic liver disease. Methods Eighteen Sprague-Dawley rats were divided into 3 groups: group A (blank control group), group B (model NS) and group C (model SAMe), 6 rats in each group. The rats in each group were treated 72 hours after intraperitoneal injection. The liver serological indexes were detected by automatic biochemical analyzer, and liver tissues were stained by HE staining and MASson staining to observe the changes of liver histopathology and collagen content. Results the serum levels of glutamic acid aminotransferase (alt), aspartate aminotransferase (AST), total bilirubin (TBiL), direct bilirubin (DBiL), indirect bilirubin (IBiL), glutamyl transpeptidase (纬 -GGT), alkaline phosphatase (ALP), total bile acid (TBA) were significantly lower in group C rats. There were significant differences between the three groups (P0.05), but there was no significant difference in the level of total cholesterol (Chol) between the three groups (P0.05). The content of collagen fibers and the area ratio of collagen fibers in group C were significantly lower than those in group B (P0.05). The difference was statistically significant (P0.05). Conclusion SAMe can further protect liver tissue by improving liver function, reducing the content and area of collagen fibers in patients with drug-induced cholestatic liver disease.
【作者单位】：南方医科大学附属珠江医院消化内科;
【分类号】：R575

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