IRE1α和IRE1β在DSS诱导的小鼠慢性结肠炎中的表达及意义

发布时间：2018-07-07 20:29

本文选题：炎症性肠病 + 慢性结肠炎　；参考：《安徽医科大学学报》2017年04期

【摘要】：目的探讨肌醇需求酶1(IRE1)α和IRE1β在葡聚糖硫酸钠(DSS)诱导的小鼠慢性结肠炎中的表达及意义。方法选择8~10周龄的C57BL/6雌性小鼠随机分为两组:对照组10只,正常饮水;慢性炎症组10只,先给予1.0%DSS自由饮用7 d,然后正常饮水14 d如此作为一个周期,循环3个周期建立小鼠慢性结肠炎模型。通过疾病活动指数(DAI)评分、结肠长度测定和HE染色等方法评估肠道炎症程度。采用实时定量PCR技术检测小鼠结肠组织中炎症因子、内质网应激因子IRE1α、IRE1β、非剪接型X-盒结合蛋白1(XBP1u)、剪接型X-盒结合蛋白1(XBP1s)和黏蛋白(MUC)2 mRNA表达,免疫组织化学方法检测IRE1α、IRE1β和MUC2表达。结果对照组小鼠无肠炎表现,而慢性炎症组在3个周期末结肠出现炎症表现。实时定量PCR结果显示白介素(IL)-6、IL-8和肿瘤坏死因子(TNF)-αmRNA在慢性炎症组高于对照组(P0.05);XBP1s和IRE1αmRNA在两组表达无明显差异;XBP1u mRNA在慢性炎症组表达低于对照组(P0.05)。IRE1β和MUC2 mRNA在慢性炎症组表达均低于对照组(P0.05)。IRE1α蛋白主要表达于结肠上皮的刷状缘、浆细胞等细胞的胞质中,其表达量在炎症组高于对照组,IRE1β蛋白和MUC2蛋白主要表达于结肠上皮细胞胞质中,两者表达量在炎症组低于对照组(P0.05)。结论 IRE1α可能通过对XBP1进行剪接来参与肠道炎症反应,并且IRE1α还有可能通过其他机制来调节自身的表达来参与炎症过程;IRE1β和MUC2可能与结肠上皮的功能维持有关,在肠道炎症过程中IRE1β和MUC2表达功能受到抑制而影响炎症的发生和发展。
[Abstract]:Objective to investigate the expression and significance of IRE1 伪 and IRE1 尾 in chronic colitis induced by dextran sulfate (DSS) in mice. Methods female C57BL / 6 mice aged 8 ~ 10 weeks were randomly divided into two groups: control group (n = 10), chronic inflammation group (n = 10), and chronic inflammation group (n = 10) were given 1.0DSS for 7 days and then normal drinking for 14 days as a cycle. The model of chronic colitis in mice was established by three cycles of circulation. The severity of intestinal inflammation was evaluated by disease activity index (Dai), colonic length and HE staining. The expression of inflammatory factors, endoplasmic reticulum stress factor IRE1 伪, non-splicing X-BP1u, splicing XBP1s and mucin 2 mRNA were detected by real-time quantitative PCR. The expressions of IRE1 伪 and MUC2 were detected by immunohistochemistry. Results there was no colitis in the control group, while in the chronic inflammation group, the colonic inflammation appeared at the end of 3 cycles. The results of real-time quantitative PCR showed that the expression of IL-8 and TNF-伪 mRNA in chronic inflammation group was higher than that in control group (P0.05). There was no significant difference in the expression of XBP1s and IRE1 伪 mRNA in chronic inflammation group. The expression of XBP1u mRNA in chronic inflammation group was lower than that in control group (P0.05). IRE1 尾 and MUC2 mRNA in chronic inflammation group. The expression of IRE1 伪 protein was significantly lower than that of the control group (P0.05). IRE1 伪 protein was mainly expressed in the brush margin of colonic epithelium. The expression of IRE1 尾 protein and MUC2 protein in the inflammatory group was higher than that in the control group, and the expression of both proteins in the cytoplasm of the inflammatory group was lower than that in the control group (P0.05). Conclusion IRE1 伪 may participate in intestinal inflammation by splicing XBP1, and IRE1 伪 may be involved in the process of inflammation by regulating its own expression. IRE1 尾 and MUC2 may be related to the maintenance of colon epithelium. The expression of IRE1 尾 and MUC2 was inhibited during intestinal inflammation, which affected the occurrence and development of inflammation.
【作者单位】：河南科技大学临床医学院河南科技大学第一附属医院消化内科;河南科技大学临床医学院河南科技大学第一附属医院肝胆外科;河南科技大学临床医学院河南科技大学第一附属医院检验科;首都医科大学附属北京康复医院消化内科;
【基金】：国家自然科学基金(编号:81370487)
【分类号】：R574.62

【相似文献】