MTTP基因rs1800591位点多态性与非酒精性脂肪性肝病的相关性研究

发布时间：2018-07-12 12:48

本文选题：微粒体甘油三酸酯转移蛋白 + 单核苷酸多态性　；参考：《中国肝脏病杂志(电子版)》2015年03期

【摘要】：目的研究微粒体甘油三酸酯转移蛋白(MTTP)基因rs1800591位点多态性与非酒精性脂肪性肝病(NAFLD)的关系。方法采用聚合酶链式反应(PCR)-限制性片段长度多态性检测219例NAFLD患者和210例正常对照组MTTP基因rs1800591位点基因型和等位基因。应用方差分析、卡方检验、非条件Logistic回归分析等方法判断NAFLD患者与正常对照组的各项指标。结果 NAFLD组与对照组两者rs1800591位点的基因型分布和等位基因频率差异有统计学意义(χ2=9.007,P=0.011;χ2=9.599,P=0.002)。经混杂因素校正后,非条件Logistic回归分析结果显示,与TT基因型相比,GG+GT基因型发生NAFLD的风险比值比:OR=1.623,95%CI为1.070~1.648,P=0.030。等位基因G携带者患NAFLD的风险是T等位基因的1.521倍(OR=1.521,95%CI为1.089~2.123,P=0.014)。TT基因型与GG+GT组ALT、LDL、TG的差异有统计学意义(ALT:P=0.001;LDL:P=0.008;TG:P=0.024)。结论青岛地区汉族人群中MTTP基因rs1800591位点多态性与患NAFLD的风险有相关性。
[Abstract]:Objective to study the relationship between rs1800591 polymorphism of microsomal triglyceride transfer protein (MTTP) gene and nonalcoholic fatty liver disease (NAFLD). Methods polymerase chain reaction (PCR) restriction fragment length polymorphism was used to detect the genotype and allele of rs1800591 loci of MTTP gene in 219 cases of NAFLD and 210 normal controls. Variance analysis, chi square test and unconditional Logistic regression analysis were used to determine the indexes of NAFLD patients and normal controls. Results the genotype distribution and allele frequency differences of rs1800591 loci between the NAFLD group and the control group were statistically significant (x 2=9.007, P= 0.011; Chi 2=9.599, P=0.002). The conditional Logistic regression analysis showed that compared with the TT genotype, the risk ratio Ratio of NAFLD in the GG+GT genotype was 1.070~1.648, and the risk of NAFLD in P=0.030. allele G carriers was 1.521 times that of the T allele (OR=1.521,95%CI 1.089~2.123, 0.014), and the difference was statistically significant. Significance (ALT:P=0.001; LDL:P=0.008; TG:P=0.024). Conclusion the polymorphism of MTTP gene rs1800591 loci is associated with the risk of NAFLD in Qingdao Han population.
【作者单位】：青岛大学医学院;青岛市市立医院;
【基金】：国家自然科学基金资助项目(81170337/H0304) 青岛市民生科技计划项目(14-2-3-17-nsh)
【分类号】：R575

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