巨噬细胞极化在白藜芦醇抗大鼠肝脏缺血再灌注损伤中的作用

发布时间：2018-07-13 12:36

【摘要】：目的探讨巨噬细胞极化在白藜芦醇(Res)抗肝脏缺血-再灌注损伤(HIRI)中的作用。方法 24只雄性SD大鼠随机分为假手术组(S组)、缺血-再灌注组(I/R组)、Res后处理组(Res组),每组8只。建立急性HIRI模型,于再灌注6 h后收集动物血液和肝脏标本。检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(AKP)及炎症因子白细胞介素(IL)-6、肿瘤坏死因子(TNF)-α、IL-10、转化生长因子(TGF)-β的浓度,采用Real-time PCR法检测肝脏组织TNF-α及IL-10 mRNA的表达情况,采用流式细胞术分析肝脏M1、M2型巨噬细胞分布情况。结果 Res后处理可以降低肝脏功能学指标ALT、AST、AKP的血清浓度;与I/R组比较,Res组IL-10和TGF-β含量升高,而IL-6和TNF-α含量降低,同时IL-10 mRNA表达量升高而TNF-α表达量下降;流式细胞分析结果显示Res后处理可以降低M1型巨噬细胞的比例,使巨噬细胞向M2型转化。结论 Res对HIRI的保护是通过调节巨噬细胞极化发挥作用的。
[Abstract]:Objective to investigate the role of macrophage polarization in resveratrol (res) against hepatic ischemia-reperfusion injury (HIRI). Methods Twenty-four male Sprague-Dawley rats were randomly divided into sham-operated group (S group) and ischemia reperfusion group (I / R group) with 8 rats in each group. Acute HIRI model was established and blood and liver samples were collected 6 hours after reperfusion. Serum alanine aminotransferase (alt), aspartate aminotransferase (AST), alkaline phosphatase (AKP), interleukin-6 (IL-6), tumor necrosis factor- 伪 (TNF- 伪) IL-10, transforming growth factor- 尾 (TGF- 尾) were detected. The expression of TNF- 伪 and IL-10 mRNA in liver tissue was detected by real-time PCR. The distribution of M 1 M 2 macrophages in liver was analyzed by flow cytometry. Results compared with the I / R group, the levels of IL-10 and TGF- 尾 increased, the contents of IL-6 and TNF- 伪 decreased, and the expression of IL-10 mRNA increased and the expression of TNF- 伪 decreased after re treatment. The results of flow cytometry showed that re post treatment could reduce the ratio of M 1 macrophages and make macrophages transform to M 2 type. Conclusion es protects HIRI by regulating macrophage polarization.
【作者单位】：长治医学院病理生理学教研室;长治医学院;
【基金】：山西省2013年国家级大学生创新创业训练资助项目(2013083) 长治医学院2016年校级大学生创新创业训练计划项目(D2016001) 长治医学院科技启动基金资助项目(No.QDZ201520)
【分类号】：R575

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