远隔缺血后适应对大鼠小肠缺血再灌注损伤的保护作用

发布时间：2018-07-15 18:54

【摘要】：目的探讨远隔缺血后适应是否对大鼠肠道缺血再灌注损伤起保护作用。方法健康雄性昆明种大鼠24只,随机分为假手术组(Con组)、缺血再灌注组(ischemia reperfusion,IR组)和远隔缺血后适应组(remote ischemic postconditioning,RIP组)共3组,每组8只。Con组只给予解剖游离肠系膜上动脉;IR组解剖出肠系膜上动脉,在肠系膜上动脉缺血60min后再灌注120min;RIP组在肠系膜上动脉缺血20min后捆绑左下肢形成4个间隔5min的循环缺血再灌注(共40min),再进行再灌注2h。测定回肠组织中丙二醛(maleiedialdehyde,MDA)和髓过氧化物酶(myeloperoxidase,MPO)的含量;光镜下观察肠道组织形态学改变,对小肠黏膜损伤程度进行评分。结果光镜下观察IR和RIP组有明显的肠道损伤,RIP组肠道损伤程度明显较IR组轻。小肠损伤按Chiu氏评分,RIP组Chiu评分值(2.10±0.63)明显低于IR组(3.20±0.76)(P0.05)。IR和RIP组回肠组织中MDA和MPO含量明显高于Con组(均P0.05),而RIP组回肠组织中MDA及MPO含量明显低于IR组(均P0.05)。结论远隔缺血后适应对小肠缺血再灌注损伤具有显著保护作用,这种远隔器官缺血后适应可能是通过减轻活性氧损伤及炎症反应来保护肠道。
[Abstract]:Objective to investigate the protective effect of adaptation after distant ischemia on intestinal ischemia reperfusion injury in rats. Methods Twenty-four healthy male Kunming rats were randomly divided into three groups: sham operation group (Con group), ischemia reperfusion group (IR group) and (remote ischemic postconditioning group (remote ischemic group). The superior mesenteric artery was dissected by dissecting the superior mesenteric artery in the IR group, and the superior mesenteric artery was dissected in the IR group. After superior mesenteric artery ischemia (60min) and reperfusion 120 minutes after reperfusion, the left lower extremities were bound to form four 5min circulatory ischemia reperfusion (40min) after superior mesenteric artery ischemia (20min), and then reperfusion was performed for 2 h. The contents of malondialdehyde (MDA) and myeloperoxidase (MPO) in ileum tissue were measured and the intestinal tissue morphology was observed under light microscope to evaluate the degree of intestinal mucosal injury. Results the degree of intestinal injury in RIP and IR group was significantly less than that in IR group. The levels of MDA and MPO in ileum of RIP group were significantly higher than those of Con group (P0.05), and the contents of MDA and MPO in RIP group were significantly lower than those in IR group (P0.05). Conclusion adaptation after remote ischemia has a significant protective effect on intestinal ischemia-reperfusion injury. This adaptation may protect the intestinal tract by reducing reactive oxygen damage and inflammatory reaction.
【作者单位】：昆明医科大学第二附属医院干疗外科;
【分类号】：R574.5

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