趋化因子受体CX3CR1在重症胰腺炎大鼠胰腺及受累器官组织中的表达及意义
发布时间：2018-07-18 08:00
【摘要】：目的通过建立大鼠重症急性胰腺炎(severe acute pancreatitis,SAP)动物模型,研究趋化因子受体CX3CR1在SAP大鼠模型胰腺组织及相关受累器官组织中的表达,探讨其能否作为SAP发生的预测指标。方法60只健康成年雄性Sprague-Dawley大鼠,体重200-250g,随机分为假手术(Sham operation,SO)组(n=20)、轻型急性胰腺炎(mild acute pancreatitis,MAP)组(n=20)及SAP组(n=20),分别以0.5%及5%牛磺胆酸钠建立MAP及SAP动物模型,于制模后6 h、12 h、24 h及48 h处死大鼠。光镜下观察各组大鼠胰腺组织病理学表现;采用速率法检测各组大鼠血清淀粉酶水平;应用酶联免疫吸附试验(Enzyme-linked immunosorbent assay,ELISA)法检测各组大鼠血清中CX3CR1及TNF-α的变化;同时应用蛋白质印迹(Western blot)法及免疫组织化学法检测各组大鼠胰腺、肺脏及肾脏组织中CX3CR1蛋白的表达;分析血清CX3CR1与大鼠胰腺病理损伤评分及血清TNF-α水平之间的相关性。结果1.SAP组各时间点(6h、12h、24h、48h)大鼠胰腺组织病理学评分均较SO组及MAP组相应时间点显著升高(P0.05)。MAP组各时间点大鼠胰腺组织病理学评分较SO组各相应时间点显著升高(P0.05)。2.SAP组各时间点大鼠血清淀粉酶水平较MAP组及SO组相应时间点均显著升高(P0.05)。3.SAP组大鼠血清CX3CR1及TNF-α水平均自制模后逐渐升高,24h达高峰,之后逐渐下降。与MAP组及SO组各相应时间点比较,均显著升高(P0.05)。4.Western blot法结果显示:SAP组大鼠胰腺及肺脏组织CX3CR1蛋白表达水平自制模后逐渐升高,24h达高峰(2.19±0.26,2.24±0.26),48h(1.89±0.23,2.02±0.27)后逐渐下降。SAP组肾脏组织CX3CR1蛋白表达水平自制模后逐渐升高,48h达高峰(2.67±0.21),与MAP组及SO组相应时间点比较均显著升高(P0.05)。5.免疫组织化学法结果显示:SAP组大鼠胰腺、肺脏及肾脏组织均有CX3CR1表达,且表达水平显著高于MAP组及SO组,其中胰腺及肺脏组织中CX3CR1表达在制模后24h达高峰,肾脏组织中CX3CR1蛋白表达在制模后48h达高峰。6.相关性分析结果:Spearman等级相关分析表明SAP组血清CX3CR1水平与胰腺病理损伤评分呈正相关(r=0.711,P0.01);Pearson相关分析表明SAP组血清CX3CR1水平与血清TNF-α水平呈正相关(r=0.828,P0.01)。结论1.趋化因子受体CX3CR1参与大鼠SAP发生发展过程,且其血清水平与胰腺组织病理损伤严重程度有关,可能能作为预测急性胰腺炎严重程度的有效指标之一,值得进一步深入研究。2.CX3CR1可能参与大鼠SAP继发急性肺损伤(acute lung injury,ALI)过程。3.CX3CR1可能参与大鼠SAP继发急性肾损伤(acute kidney injury,AKI)过程。
[Abstract]:Objective to study the expression of chemokine receptor (CX3CR1) in pancreatic tissues and related involved organs of severe acute pancreatitis (SAP) in rats, and to explore whether it can be used as a predictor for the occurrence of severe acute pancreatitis (SAP). Methods Sixty healthy adult male Sprague-Dawley rats, weighing 200-250 g, were randomly divided into three groups: sham operation group (n = 20), mild acute pancreatitis (map) group (n = 20) and SAP group (n = 20). Map and SAP animal models were established with 0.5% and 5% sodium taurocholate, respectively. The rats were sacrificed at 6 h, 12 h, 24 h and 48 h. The histopathological manifestations of pancreas were observed under light microscope, the serum amylase level was detected by rate method, and the changes of CX3CR1 and TNF- 伪 in serum were detected by Enzyme-linked immunosorbent assay-ELISA (Elisa). Western blot and immunohistochemistry were used to detect the expression of CX3CR1 protein in pancreas, lung and kidney of rats, and the correlation between serum CX3CR1 and pancreatic pathological injury score and serum TNF- 伪 level was analyzed. Results 1. The histopathological scores of pancreas in SAP group were significantly higher than those in so group and map group (P0.05). The histopathological score of SAP group was significantly higher than that of so group at each time point (P0.05). 2. SAP group had significantly higher pancreatic histopathological score than that of so group at each time point (P0.05). The levels of serum amylase in SAP group were significantly higher than those in map group and so group (P0.05). 3. The levels of CX3CR1 and TNF- 伪 in SAP group were increased gradually and reached the peak at 24 h after self-made model. Then it gradually declined. Compared with map group and so group, The expression level of CX3CR1 protein in pancreas and lung tissues of rats in the SAP group was significantly increased (P0.05). 4. The expression level of CX3CR1 protein in the pancreas and lung tissues of the rats in the SAP group gradually increased to a peak of (2.19 卤0.26) 2.24 卤0.26 after 24 hours and gradually decreased after 48 hours (1.89 卤0.23 卤2.02 卤0.27). The expression level of CX3CR1 protein in the renal tissue of SAP group gradually increased after 48 hours. The peak value was (2.67 卤0.21), which was significantly higher than that in map group and so group (P0.05). The expression of CX3CR1 in pancreas, lung and kidney of rats in SAP group was significantly higher than that in map group and so group. The expression of CX3CR1 in pancreas and lung reached its peak 24 hours after modeling. The expression of CX3 CR1 protein reached its peak at 48h after modeling. The correlation analysis showed that the serum CX3CR1 level was positively correlated with the pancreatic pathological injury score in SAP group. Pearson correlation analysis showed that there was a positive correlation between serum CX3CR1 level and serum TNF- 伪 level in SAP group (r = 0.828, P 0.01). Conclusion 1. The chemokine receptor CX3CR1 is involved in the pathogenesis and development of SAP in rats. The serum level of CX3CR1 is related to the severity of pancreatic pathological injury. CX3CR1 may be one of the effective indicators to predict the severity of acute pancreatitis. 2. CX3CR1 may be involved in the process of acute lung injury (acute lung injuryto Ali). 3. CX3CR1 may be involved in the process of acute renal injury (acute kidney injura).
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R576
[Abstract]:Objective to study the expression of chemokine receptor (CX3CR1) in pancreatic tissues and related involved organs of severe acute pancreatitis (SAP) in rats, and to explore whether it can be used as a predictor for the occurrence of severe acute pancreatitis (SAP). Methods Sixty healthy adult male Sprague-Dawley rats, weighing 200-250 g, were randomly divided into three groups: sham operation group (n = 20), mild acute pancreatitis (map) group (n = 20) and SAP group (n = 20). Map and SAP animal models were established with 0.5% and 5% sodium taurocholate, respectively. The rats were sacrificed at 6 h, 12 h, 24 h and 48 h. The histopathological manifestations of pancreas were observed under light microscope, the serum amylase level was detected by rate method, and the changes of CX3CR1 and TNF- 伪 in serum were detected by Enzyme-linked immunosorbent assay-ELISA (Elisa). Western blot and immunohistochemistry were used to detect the expression of CX3CR1 protein in pancreas, lung and kidney of rats, and the correlation between serum CX3CR1 and pancreatic pathological injury score and serum TNF- 伪 level was analyzed. Results 1. The histopathological scores of pancreas in SAP group were significantly higher than those in so group and map group (P0.05). The histopathological score of SAP group was significantly higher than that of so group at each time point (P0.05). 2. SAP group had significantly higher pancreatic histopathological score than that of so group at each time point (P0.05). The levels of serum amylase in SAP group were significantly higher than those in map group and so group (P0.05). 3. The levels of CX3CR1 and TNF- 伪 in SAP group were increased gradually and reached the peak at 24 h after self-made model. Then it gradually declined. Compared with map group and so group, The expression level of CX3CR1 protein in pancreas and lung tissues of rats in the SAP group was significantly increased (P0.05). 4. The expression level of CX3CR1 protein in the pancreas and lung tissues of the rats in the SAP group gradually increased to a peak of (2.19 卤0.26) 2.24 卤0.26 after 24 hours and gradually decreased after 48 hours (1.89 卤0.23 卤2.02 卤0.27). The expression level of CX3CR1 protein in the renal tissue of SAP group gradually increased after 48 hours. The peak value was (2.67 卤0.21), which was significantly higher than that in map group and so group (P0.05). The expression of CX3CR1 in pancreas, lung and kidney of rats in SAP group was significantly higher than that in map group and so group. The expression of CX3CR1 in pancreas and lung reached its peak 24 hours after modeling. The expression of CX3 CR1 protein reached its peak at 48h after modeling. The correlation analysis showed that the serum CX3CR1 level was positively correlated with the pancreatic pathological injury score in SAP group. Pearson correlation analysis showed that there was a positive correlation between serum CX3CR1 level and serum TNF- 伪 level in SAP group (r = 0.828, P 0.01). Conclusion 1. The chemokine receptor CX3CR1 is involved in the pathogenesis and development of SAP in rats. The serum level of CX3CR1 is related to the severity of pancreatic pathological injury. CX3CR1 may be one of the effective indicators to predict the severity of acute pancreatitis. 2. CX3CR1 may be involved in the process of acute lung injury (acute lung injuryto Ali). 3. CX3CR1 may be involved in the process of acute renal injury (acute kidney injura).
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R576
【相似文献】
相关期刊论文 前10条
1 陈同度,张昌颖;素食大鼠的贫血现象[J];营养学报;1957年04期
2 陈伟强;赵善广;;自制注射用大鼠固定装置[J];上海实验动物科学;1992年04期
3 肖柳英,林培英,冯昭明,张丹;不同周龄的SD大鼠生理、生化及体重的正常值测定[J];中药新药与临床药理;1996年03期
4 李淑云;简易大鼠灌胃器的制作[J];锦州医学院学报;2001年04期
5 杨明智,陈积圣;一种大鼠抓取与固定的新工具介绍[J];上海实验动物科学;2001年03期
6 戴英,陆群;复方H_(505)对Wistar大鼠外周血的血液流变学指标的影响[J];中国血液流变学杂志;2001年01期
7 韦应波,孙喜庆,曹新生,姚永杰,冯岱雅,杨长斌;+Gz暴露时间对大鼠记忆功能和行为的影响[J];航天医学与医学工程;2003年01期
8 吕学军,郭俊生,李敏,周利梅,张永娟;晕船大鼠体内铁含量的变化[J];中国职业医学;2003年04期
9 汤仁仙,王迎伟,王慧,周峰;201A中药合剂对大鼠抗肾小球基底膜肾炎病变的影响[J];徐州医学院学报;2003年06期
10 孙同柱,付小兵,翁立新,梁雪梅,陈伟;介绍一种简易的大鼠保定方法[J];上海实验动物科学;2004年01期
相关会议论文 前10条
1 尹音;孙振宇;胡敏;李冬霞;;持续性高正加速度对大鼠颞颌关节损伤的作用[A];第八届全国颞下颌关节病学及（牙合）学大会论文汇编[C];2011年
2 祝~=骧;iJ梊霞;洃克琴;崔素英;文允摪;，
本文编号：2131259
本文链接：https://www.wllwen.com/yixuelunwen/xiaohjib/2131259.html
最近更新
教材专著
