脂肪间充质干细胞经肝动脉移植治疗晚期肝病
发布时间：2018-07-20 18:49
【摘要】：背景:干细胞移植是晚期肝病比较有前景的治疗方法,脂肪间充质干细胞是继骨髓间充质干细胞后的又一研究热点。目的:探讨经肝动脉移植大鼠脂肪间充质干细胞对晚期肝病的影响。方法:将45只SD大鼠随机分为3组,对照组、模型组和移植组各15只。模型组和移植组皮下注射四氯化碳诱导建立大鼠肝硬化模型,移植组大鼠经肝动脉注入1 mL CFSE标记的脂肪间充质干细胞,模型组经肝动脉注入等量的生理盐水,对照组不予处理。移植治疗后4周,观察各组大鼠的肝脏病理组织学变化,肝功能及肝纤维化程度。结果与结论:1在大鼠的肝脏内见到绿色荧光标记的脂肪间充质干细胞。2经苏木精-伊红染色和Masson染色后发现:模型组大鼠肝小叶结构不清,有假小叶形成,细胞浊肿疏松,部分变性坏死,见炎症细胞浸润;对照组大鼠肝组织未见异常。移植组大鼠的肝脏病理组织学改变介于模型组和对照组之间。3模型组大鼠的血清白蛋白水平低于对照组(P0.05),胆红素、转氨酶和Ⅳ型胶原水平明显高于对照组(P0.05);移植组大鼠的血清白蛋白水平高于模型组(P0.05),胆红素、转氨酶和Ⅳ型胶原低于模型组(P0.05)。4由此可见脂肪间充质干细胞移植治疗可以改善肝硬化大鼠的肝脏功能,减轻肝纤维化程度。
[Abstract]:Background: stem cell transplantation is a promising treatment for advanced liver disease. Adipose mesenchymal stem cells are another research hotspot after bone marrow mesenchymal stem cells. Objective: to investigate the effect of transhepatic artery transplantation of adipose mesenchymal stem cells on advanced liver disease in rats. Methods: 45 SD rats were randomly divided into 3 groups: control group, model group and transplantation group. The liver cirrhosis model was induced by subcutaneous injection of carbon tetrachloride into the model group and the transplantation group. Rats in the transplantation group were injected with 1 mL CFSE labeled adipose mesenchymal stem cells through the hepatic artery, and the model group were injected with the same amount of normal saline through the hepatic artery. The control group was not treated. Liver histopathology, liver function and liver fibrosis were observed 4 weeks after transplantation. Results and conclusion the green fluorescent labeled adipose mesenchymal stem cells were found in the liver of rats by hematoxylin eosin staining and Masson staining. The results showed that the hepatic lobules in the model group were not clear, pseudolobules were formed, and the cells were turbid and loose. Partial degeneration and necrosis, inflammatory cell infiltration, and no abnormal liver tissue were found in the control group. The liver histopathological changes of the transplantation group were between the model group and the control group. 3. The serum albumin level of the model group was lower than that of the control group (P0.05), bilirubin, bilirubin, The levels of transaminase and type 鈪，
本文编号：2134431
[Abstract]:Background: stem cell transplantation is a promising treatment for advanced liver disease. Adipose mesenchymal stem cells are another research hotspot after bone marrow mesenchymal stem cells. Objective: to investigate the effect of transhepatic artery transplantation of adipose mesenchymal stem cells on advanced liver disease in rats. Methods: 45 SD rats were randomly divided into 3 groups: control group, model group and transplantation group. The liver cirrhosis model was induced by subcutaneous injection of carbon tetrachloride into the model group and the transplantation group. Rats in the transplantation group were injected with 1 mL CFSE labeled adipose mesenchymal stem cells through the hepatic artery, and the model group were injected with the same amount of normal saline through the hepatic artery. The control group was not treated. Liver histopathology, liver function and liver fibrosis were observed 4 weeks after transplantation. Results and conclusion the green fluorescent labeled adipose mesenchymal stem cells were found in the liver of rats by hematoxylin eosin staining and Masson staining. The results showed that the hepatic lobules in the model group were not clear, pseudolobules were formed, and the cells were turbid and loose. Partial degeneration and necrosis, inflammatory cell infiltration, and no abnormal liver tissue were found in the control group. The liver histopathological changes of the transplantation group were between the model group and the control group. 3. The serum albumin level of the model group was lower than that of the control group (P0.05), bilirubin, bilirubin, The levels of transaminase and type 鈪，
本文编号：2134431
本文链接：https://www.wllwen.com/yixuelunwen/xiaohjib/2134431.html
最近更新
教材专著
