纤溶酶原激活物抑制剂-1在大鼠肝纤维化模型及自发逆转肝组织中的动态变化及意义

发布时间：2018-07-21 10:08

【摘要】：目的 :探讨纤溶酶原激活物抑制剂-1(plasminogen activator inhibitor-1,PAI-1)在肝纤维化发生、发展及消退过程中的表达变化及意义。方法:采用皮下注射四氯化碳(carbon tetrachloride,CCl4)方法制备大鼠肝纤维化模型,在注射及停止注射后不同时间,取组织标本,HE和Van Gieson氏(简称VG)染色。明确纤维化分期后,利用免疫组化及RT-PCR方法,观察PAI-1在肝纤维化进程及逆转中表达变化情况。结果:在正常大鼠肝脏中,PAI-1仅在汇管区细胞浆有少量表达;在纤维化肝脏,PAI-1主要分布于肝血窦壁及细胞浆。随着纤维化的进展,PAI-1表达量进行性增加(正常对照组为0.142±0.030,模型组注射CCl42、6和8周组分别为0.361±0.048、0.757±0.068和0.838±0.048);肝纤维化自然消退过程中又逐渐减弱(自发逆转2、4和6周组分别为0.613±0.054、0.524±0.060和0.210±0.044)。RT-PCR检测,模型组注射CCl42、6和8周后,PAI-1 m RNA在肝脏组织中的表达分别是正常肝组织的(6.83±2.60)倍、(12.43±2.65)倍和(26.32±5.17)倍,停止注射CCl4后,在自发逆转2、4和6周时,PAI-1 m RNA在肝脏组织中的表达只是正常肝组织中的(17.86±4.60)倍、(14.62±5.99)倍和(11.21±1.98)倍。结论:PAI-1在肝纤维化进程中持续上调,在肝纤维化逆转过程中表达下调,可能在肝纤维化发生发展中起重要作用。
[Abstract]:Aim: to investigate the expression and significance of plasminogen activator inhibitor 1 (PAI-1) in the process of hepatic fibrosis. Methods: a rat model of hepatic fibrosis was established by subcutaneous injection of carbon tetrachloride (carbon tetrachloride CCl4). The specimens were stained with HE and Van Gieson's (VG) staining at different time points after injection or discontinuation of the injection. The expression of PAI-1 in the progression and reversal of hepatic fibrosis was observed by immunohistochemistry and RT-PCR. Results: in the normal rat liver, PAI-1 was expressed only in the cytoplasm of the portal area and mainly in the sinusoidal wall and cytoplasm of the fibrotic liver. With the progression of fibrosis, the expression of PAI-1 increased progressively (0.142 卤0.030 in the normal control group, 0.361 卤0.048 卤0.757 卤0.068 and 0.838 卤0.048 in the model group at week 8 and 0.361 卤0.048 in the model group, respectively), and gradually decreased during the spontaneous regression of hepatic fibrosis (0.613 卤0.050.524 卤0.060 and 0.210 卤0.044 in the spontaneous reversion of 24 and 6 weeks, respectively). The expression of PAI-1 mRNA in the model group was (6.83 卤2.60) times, (12.43 卤2.65) times and (26.32 卤5.17) times of the normal liver tissue, respectively. The expression of PAI-1 mRNA in the liver was (17.86 卤4.60) times, (14.62 卤5.99) and (11.21 卤1.98) times of the normal liver tissue. Conclusion: the expression of 1% PAI-1 is up-regulated and down-regulated in the process of hepatic fibrosis, which may play an important role in the occurrence and development of liver fibrosis.
【作者单位】：南京医科大学附属无锡第二人民医院消化内科;第二军医大学长征医院消化内科;
【基金】：无锡市医管中心联合公关项目(YGZX1202)
【分类号】：R575.2

【参考文献】