超声监测非酒精性脂肪肝兔模型的建立

发布时间：2018-08-03 12:13

【摘要】：目的脂肪肝的发病率逐步增高。文中拟建立一种可供研究的非酒精性脂肪肝兔模型,研究其超声表现。方法将40只日本大耳兔按随机数字表法分为对照组和实验组,各20只,对照组给予普通饲料,实验组给予高脂饮食(胆固醇2%,牛油10%,白糖5%,蛋黄粉8%,基础饲料75%)喂养。在0、12、16、20周时超声观察各组兔肝,20周末处死动物,取出兔肝,进行病理观察。结果超声和病理均显示非酒精性脂肪肝兔造模成功。造模12、16、20周时实验组兔体重明显高于对照组[(2.43±0.17)kg vs(2.25±0.18)kg,(3.04±0.25)kg vs(2.56±0.13)kg,(3.55±0.22)kg vs(3.10±0.13)kg,P㩳0.05]。超声观察12周时便有脂肪肝表现,实验组19只兔中8例为轻度脂肪肝,9例为中度,另2例为重度。随着喂饲期的延长,脂肪肝程度逐渐加重,16周以中度脂肪肝回声为主,20周以中重度脂肪肝回声为主,10例为中度,9例为重度,且3例有肝硬化腹水的表现。病理观察与超声结果一致,并可见纤维化表现。结论可通过高脂饮食建立良好的非酒精性脂肪肝兔模型,超声是监测脂肪肝形成过程的可靠手段。
[Abstract]:Objective to increase the incidence of fatty liver. A non-alcoholic fatty liver model was established in this paper. Methods Forty Japanese big ear rabbits were randomly divided into control group (n = 20) and experimental group (n = 20). The control group (n = 20) was given common diet, the experimental group was fed with high-fat diet (cholesterol 2, butter 10, sugar 5, egg yolk powder 8, basic feed 75%). The rabbits were killed at the 20th week after 20 weeks of ultrasound observation, and the rabbits' liver was taken out for pathological observation. Results Ultrasound and pathology showed that the model of non-alcoholic fatty liver was successful. The body weight of the experimental group was significantly higher than that of the control group (2.43 卤0.17) kg vs (2.25 卤0.18) kg, (2.04 卤0.25) kg vs (2.56 卤0.13) kg, (3.55 卤0.22) kg vs (3.10 卤0.13 kg) at 20 weeks. In the experimental group, 8 cases were mild fatty liver disease, 9 cases were mild fatty liver disease, and 2 cases were severe. With the prolongation of feeding period, the degree of fatty liver was aggravated gradually in 16 weeks. In 20 weeks, the degree of fatty liver was mainly moderate fatty liver echo. In 20 weeks, 10 cases were moderate fatty liver echo, 9 cases were severe, and 3 cases had ascites of liver cirrhosis. Pathological findings were consistent with ultrasound findings, and fibrosis could be seen. Conclusion A good non-alcoholic fatty liver model can be established by high fat diet, and ultrasound is a reliable method to monitor the formation of fatty liver.
【作者单位】：昆明医科大学第四附属医院;云南省第二人医院超声科;
【基金】：云南省卫生厅卫生系统学科带头人培养计划基金(D-201204) 云南省中青年学术技术带头人后备人才基金(2010CI032) 云南省科技计划项目(2012FB081)
【分类号】：R575.5;R445.1

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