非糜烂性反流病患者食管上皮细胞钙黏蛋白的表达及细胞间隙的改变

发布时间：2018-08-03 16:46

【摘要】：目的探讨非糜烂性反流病(NERD)患者食管上皮细胞钙黏蛋白(E-cadherin)的表达变化及细胞间隙的改变。方法采用免疫组化染色法检测43例份NERD食管下段上皮组织与26例正常食管下段上皮组织中的Ecadherin,在光镜下观察切片并采集图像,采用图像分析系统测量细胞间隙。结果 E-cadherin在NERD食管上皮组织中异常表达36例(失表达4例、不完整性表达32例),异常表达率83.7%;在正常食管上皮组织中异常表达5例(失表达0例、不完整性表达5例),异常表达率19.2%;二者相比,P0.01。NERD患者食管鳞状上皮细胞间隙[(1.23±0.04)μm]大于正常食管鳞状上皮细胞间隙[(0.68±0.06)μm],二者相比,P0.01。结论 E-cadherin在NERD患者食管上皮组织中低表达,导致细胞间隙增宽,可能是NERD发病的机制之一。
[Abstract]:Objective to investigate the expression of cadherin (E-cadherin) and the change of intercellular space in esophageal epithelial cells from patients with non erosive reflux disease (NERD). Methods E-cadherin in 43 cases of lower esophageal epithelium of NERD and 26 cases of normal lower esophageal epithelium were detected by immunohistochemical staining. The sections were observed under light microscope and the images were collected. The intercellular space was measured by image analysis system. Results the abnormal expression of E-cadherin was found in 36 cases (4 cases lost expression, 32 cases incomplete expression) in NERD esophagus, and 5 cases (0 cases) in normal esophageal epithelium, the abnormal expression rate was 83.7%. The abnormal expression rate was 19.2% in 5 cases of incomplete expression, and the gap of esophageal squamous epithelium [(1.23 卤0.04) 渭 m] in patients with P0.01.NERD was higher than that in normal esophageal squamous epithelium [(0.68 卤0.06) 渭 m], and the difference between the two groups was (0.68 卤0.06) 渭 m. Conclusion the low expression of E-cadherin in esophageal epithelium of NERD patients may be one of the mechanisms of NERD.
【作者单位】：扬州大学临床医学院扬州苏北人民医院;扬州大学医学院;
【分类号】：R571

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